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Basic and Clinical Neuroscience، جلد ۹، شماره ۶، صفحات ۵-۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Different Expressions of Specific Transcription Factors of Th1 (T-bet) and Th2 cells (GATA-3) by Peripheral Blood Mononuclear Cells From Patients With Multiple Sclerosis |
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| چکیده انگلیسی مقاله |
Introduction: Multiple Sclerosis (MS) is an inflammatory disorder caused by self-reactive Th1 lymphocytes, while Th2 cells may confer protection. The Th1 and Th2 cell differentiation are regulated by specific transcription factors, especially T-bet and GATA-3, respectively. This investigation aimed to measure the T-bet and GATA-3 expression by Peripheral Blood Mononuclear Cells (PBMCs) obtained from MS patients after specific and non-specific in vitro stimulation. Methods: The PBMCs were separated from 22 patients with MS and 20 healthy individuals. They were cultured at 37°C for 24 h in the absence of a stimulator or in the presence of Myelin oligodendrocyte Glycoprotein (MOG) or Phytohemagglutinin (PHA) at a concentration of 10 μg/mL. Then the T-bet and GATA-3 expression was measured by real time-PCR. Results: The T-bet expression was enhanced, while the GATA-3 expression diminished. Therefore the expression of T-bet/GATA-3 ratio diminished in not-stimulated, MOG-stimulated and PHA-stimulated PBMCs from MS patients compared with equal cultures from the healthy individuals (P< 0.01, P< 0.01 and P< 0.01, for T-bet; P< 0.03, P< 0.01 and P< 0.02, for GATA-3; P< 0.01, P< 0.001 and P< 0.01 for T-bet/GATA-3 ratio, respectively). The not-stimulated, MOG-stimulated, and PHA-stimulated PBMCs from men with MS expressed higher amounts of GATA-3 than equal cells from MS women (P< 0.05, P< 0.05 and P< 0.01, respectively). Conclusion: These results probably indicate an imbalance in Th1/Th2 cells in the level of transcription factors with a tendency toward Th1 cells in MS. The clinical utilization of the transcription factors as novel biomarkers of MS should be evaluated in further studies. |
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| کلیدواژه‌های انگلیسی مقاله |
Multiple Sclerosis, Th1, Th2, T-bet, GATA-3 |
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| نویسندگان مقاله |
| Zahra Etesam
Neuroscience Research Center, Department of Neurology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Maryam Nemati
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Mohammad-Amin Ebrahimizadeh
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Hossain-Ali Ebrahimi
Neuroscience Research Center, Department of Neurology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Hossain Hajghani
Department of Immunology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Tahereh Khalili
Department of Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
| Abdollah Jafarzadeh
Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-900-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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