سامانه اطلاعات پژوهشی ایران - NRIP Journals

جمعه 21 شهریور 1404


Research in Molecular Medicine، جلد ۴، شماره ۱، صفحات ۲۴-۲۹


عنوان فارسی

چکیده فارسی مقاله

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عنوان انگلیسی	An Efficient Protocol for Embryonic Carcinoma Cells P19 Differentiation to Cardiomyocytes Using Oxytocin as Inducer

چکیده انگلیسی مقاله	Background: The capability of embryonic carcinoma cells P19 in differentiation to Cardiomyocyte was examined through inducing effects of Oxytocin (OT) and 5-Azacytidin (5Az) individually and compared with each other in laboratory condition. Materials and Methods: P19 Embryoid Bodies (EBs) was formed through hanging drops method. Then, EBs were treated with (5Az) or (OT) and the EB medium (Ctrl) until 12 days. Morphology and beating number per minute were recorded every two days. Viability was carried out every three days. The expression of several cardiomyocyte-associated genes was assessed by RT-PCR. Results: The beating area percentage of EBs in OT treatment groups was more than that of the 5Az group in all days of experiment. However, only in final stage, a significant increase was observed in beating area of OT group. There was no significant difference in viability and morphological changes. OT induction expressed three more specific proteins in cell culture than 5Az. Conclusion: Statistical analysis revealed that response to OT inducer was more excessive than 5Az in all treatment groups. The Oxytocin was found to be effective inducer of cardiomyocytes differentiation from embryonic carcinoma cells P19 than 5-azacytidine.

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نویسندگان مقاله	مجید ملک زاده شفارودی \| majid malekzadeh shafaroudi department of anatomy and cell biology, sari faculty medicine, mazandaran university of medical sciences, mazandaran, sari- iran, post box 48175-1665 سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences) نوراله رضایی \| nourollah rezaei department of anatomy and cell biology, sari faculty medicine, mazandaran university of medical sciences, mazandaran, sari- iran, post box 48175-1665 سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences) سعید عابدیان کناری \| saeid abedian kenari immunogenetic research center irc , sari medical faculty, mazandaran university of medical sciences, mazandaran; sari- iran, post box 48175-1665 سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences) مریم نظم بجنوردی \| maryam nazm bojnordi department of anatomy and cell biology, sari faculty medicine, mazandaran university of medical sciences, mazandaran, sari- iran, post box 48175-1665 سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences) مصطفی لطیف پور \| mostafa latifpour department of anatomy, tehran medical faculty, tehran university of medical sciences, tehran- iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences) هاتف قاسمی حمیدآبادی \| hatef ghasemi hamidabadi department of anatomy and cell biology, sari medical faculty, mazandaran university of medical sciences, mazandaran, sari, iran, post box 48175-1665 سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

نشانی اینترنتی	http://rmm.mazums.ac.ir/browse.php?a_code=A-10-191-1&slc_lang=en&sid=en
فایل مقاله	فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده	en
موضوعات مقاله منتشر شده	داروسازی
نوع مقاله منتشر شده	پژوهشی

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