سامانه اطلاعات پژوهشی ایران - NRIP Journals

پنجشنبه 20 شهریور 1404


Research in Molecular Medicine، جلد ۴، شماره ۱، صفحات ۳۰-۳۵


عنوان فارسی

چکیده فارسی مقاله

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عنوان انگلیسی	Polyclonal Antibody Production against Mouse Purified IgG2a towards Use in Basic Research

چکیده انگلیسی مقاله	Background: The ability of polyclonal antibodies to react with many epitopes of an antigen makes them valuable reagents in research and diagnosis. The aim of this study was purification of mouse IgG2a and production of polyclonal antibody against purified mouse IgG2a subclass. Materials and Methods: Mouse IgG2a was purified by ProA affinity. Verification method of the purified antibody was SDS-PAGE and ELISA by a mouse isotyping Kit. Rabbit was immunized with purified IgG2a. The production of antibody in rabbit was investigated by direct ELISA method. Rabbit serum was collected and precipitated at the final concentration of 50% ammonium sulfate. Polyclonal antibody was purified by ion-exchange chromatography and labeled with HRP. The titre and cross reactivity of product was detected by direct ELISA method. Results: The results of SDS-PAGE in reduced and non-reduced conditions showed bands with 50-KDa, 25-30 KDa MW and a distinct band with 150 KDa MW. Isotype determination showed the presence of mouse IgG2a in related fraction. The titer of Anti-mouse polyclonal antibody was 200000. The optimum titer of prepared HRP conjugated IgG was 4000. Conjugated rabbit IgG has more cross reactivity with mouse IgG2b. Conclusion: Taking together, affinity chromatography and ion-exchange chromatography are appropriate techniques for purification of mouse IgG subclasses and rabbit IgG, respectively. .

کلیدواژه‌های انگلیسی مقاله

نویسندگان مقاله	زهرا مرادی nebrin \| zahra moradi nebrin immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) جعفر مجیدی \| jafar majidi tabriz autonomous university of medical sciences, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) لیلی عاقبتی ملکی \| leili aghebati maleki immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) توحید کاظمی \| tohid kazemi department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) جلال عبدالعلی زاده \| jalal abdolalizadeh department of immunology, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) سمیه داداشی \| somaieh dadashi immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) صادق عیوضی \| sadeg eyvazi immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) مجید احمدی \| majid ahmadi immunology research center, faculty of medicine, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences) نعیمه مجیدی ذوالبنین \| naiemeh majidi zolbanin pharmacology department, faculty of pharmacy, tabriz university of medical sciences, tabriz, iran سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی	http://rmm.mazums.ac.ir/browse.php?a_code=A-10-745-1&slc_lang=en&sid=en
فایل مقاله	فایلی برای مقاله ذخیره نشده است
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زبان مقاله منتشر شده	en
موضوعات مقاله منتشر شده	ایمونولوژی
نوع مقاله منتشر شده	پژوهشی

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