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Research in Molecular Medicine، جلد ۱، شماره ۱، صفحات ۳۶-۴۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Molecular identification of ovine Babesia spp. in north of Iran |
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| چکیده انگلیسی مقاله |
Background: Babesia is blood-tissue parasite, which are transmitted by hard ticks from Ixodidae family. The parasite is the cause of Babesiosis among ruminants. Babesia is as one of main fatal factors among livestock in endemic regions such as Iran. The aim of this study was to investigate Babesia spp infection using microscopic and molecular methods among small ruminants in Mazandaran and Golestan provinces, northern Iran, in 2011-2012. Materials and Methods: In this cross-sectional study, 22 flocks of total 220 sheep and goats were selected from different regions of these provinces. Subsequently, ten suspected animals were selected randomly and blood sample were taken from their ear. To diagnose thick and thin smears were prepared and stained with Geimsa stain and then examined under light microscope. Standard PCR and Semi nested PCR was performed to differentiate genus of Theileria and Babesia, also identify the species of Babesia. Results: A total of 220 blood samples (160 sheep and 60 goats), 34 cases (15.4 %) had Babesia infection using microscopic. Whereas, 11 cases (5%) were found positive through Babesia spp using standard PCR and also among them, two cases were found mixed infection through Theileria spp. In addition, two microscopic negative were positive by PCR assay. Totally, using semi-nested PCR, Babesia ovis (n=10) and Babesia motasi (n=1) were detected. Conclusion: Our results showed ovine Babesiosis was common in the Iranian Northern provinces .Moreover, Babesia ovis is the main causative agent of ovine Babesiosis in this areas. The relatively high prevalence of Babesia infection in livestock indicates the epizootic stability status of babesiosis in the northern part of Iran. |
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| نویسندگان مقاله |
سید موسی متولی حقی | seyed mousa motavalli haghi
studentresearchcommittee, school of medicine, mazandaranuniversity ofmedicalsciences sari, iran
مهدی فکار | mahdi fakar
molecular and cell biology research center, mazandaran university of medical sciences, sari, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
مهدی شریف | mehdi sharif
toxoplasmosis research center, mazandaran university of medical sciences, sari, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
عبدالستار پقه | abdolsatar paghe
toxoplasmosis research center, mazandaran university of medical sciences, sari, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
میترا شربت خوری | mitra sharbatkhori
infectious diseases research center, golestan university of medical sciences, gorgan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی گلستان (Golestan university of medical sciences)
رشید توکلی | rashid tavakoli
veterinarylaborator,veterinary network of golestanprovince
شیرزاد غلامی | shirzad gholami
toxoplasmosis research center, mazandaran university of medical sciences, sari, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-5&slc_lang=en&sid=en |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
انگل شناسی |
| نوع مقاله منتشر شده |
پژوهشی |
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