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Iranian Journal of Veterinary Medicine، جلد ۷، شماره ۱، صفحات ۱۵-۲۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
The mRNAexpression study on small amount of Theileria annulata lymph node biopsy sample using SMART-cDNA technology |
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| چکیده انگلیسی مقاله |
BACKGROUND: A major issue in many gene expressionstudies utilizing small amount of biological materials is thelimited quantity of RNApurified from clinical samples, which isoften used for RT-PCR or standard Northern blot analysis.OBJECTIVES: The SMART cDNA synthesis method and subsequentSMART-cDNA-PCR technique was used to analyse 3genes in macroschizonts of Theileria annulata in small lymphnode biopsy material. METHODS: The SMART-cDNA of TaSpgene was cloned in pTZ57R/T-vector and sequenced. We focusedon genes encoding surface proteins TaSp, TaD and HSP70.RESULTS: Our results showed that SMART cDNA dependablyreproduces the expression profile found in messenger RNA. TheRT-SMART-PCR showed the amplification of the processedmRNAs. The sequencing analysis showed that the amplifiedcDNA was coded for TaSp protein in Theileria annulata.CONCLUSIONS: It was concluded that the SMART PCRtechnique is practical for amplification of complete sequence ofmRNAs in the form of cDNAs, and therefore for gene expressionstudies if only small amounts of starting material are available. |
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| کلیدواژههای انگلیسی مقاله |
Theileria annulata,SMART-cDNA,TaSp,TaD,HSP70 |
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| نویسندگان مقاله |
نسترن صدر شیرازی | sadr shirazi
department of parasitology, faculty of veterinary medicine, university of tehran,tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
پرویز شایان |
department of parasitology, faculty of veterinary medicine, university of tehran,tehran, iran; institute of molecular biological system transfer mbst , tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
berigiteh اکرت |
institute of molecular biological system transfer mbst , tehran, iran
الهه ابراهیم زاده |
department of parasitology, faculty of veterinary medicine, university of tehran,tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
صدیقه جعفری |
department of parasitology, faculty of veterinary medicine, university of tehran,tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
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| نشانی اینترنتی |
http://ijvm.ut.ac.ir/article_32019_3e488d7a07aad80a635656ada23e1571.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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