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، جلد ۷، شماره ۳، صفحات ۷۳-۷۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Comparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens |
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| چکیده انگلیسی مقاله |
Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by Dot-ELISA using aspecific anti-brucella antibody, a suitable peroxidase conjugate and substrate. Of the 94 samples, 5 (5.31%)were positive in Dot-ELISA and 4 (4.25%) had positive cultures. In comparison with culture, the sensitivityand specificity of Dot-ELISA for detection of brucellae in the samples was 80 and 100%, respectively. Therewas 98.9% agreement between the two tests. The results indicated that Dot-ELISA is a good and rapid testwith acceptable sensitivity and specificity for detection of Brucella spp. in aborted fetal stomach contents. |
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| کلیدواژه‌های انگلیسی مقاله |
Brucellosis,Brucella spp,Dot-ELISA |
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| نویسندگان مقاله |
m قربانپور |
department of pathobiology, school of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)
محمدرضا seyfiabad شاپوری | m r seyfiabad shapouri
department of pathobiology, school of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)
s گورانی نژاد |
department of clinical sciences, school of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)
e جلالی |
graduated from school of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)
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| نشانی اینترنتی |
http://ijvr.shirazu.ac.ir/article_2653_f62a0bf466a8f7a826ea25e2105277f4.pdf |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
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