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Research in Molecular Medicine، جلد ۷، شماره ۲، صفحات ۰-۰
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عنوان فارسی |
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چکیده فارسی مقاله |
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کلیدواژههای فارسی مقاله |
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عنوان انگلیسی |
Isolation, Antimicrobial resistance profiles and virulence genotyping of Salmonella spp. from food products in Mazandaran, northern Iran |
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چکیده انگلیسی مقاله |
Background: Salmonella is the most important source of food-borne infections around the world. Human salmonellosis is also found in fresh fruit and vegetable salads. Objective: to investigate detection of Salmonella spp., in Hamburger, Vegetable salads and Cream-filled pastries from factories, supermarkets, restaurants and local confectionery, Mazandaran, Iran, as well as, to assess their pathogenic potential and antimicrobial resistance. Methods: A total of 90 samples, 30 samples of each, were randomly collected. Biochemichal and serological test performed to detect the Salmonella spp. Antimicrobial susceptibility tests were determined by the disc diffusion method and virulent genes investigated performed by polymerase chain reaction (PCR) method. Results: 54 out of 90 samples were considered as Salmonella spp. by biochemical tests. Of which 46 (85.2%) were recovered by the 16S-23S rRNA PCR test. 19 samples were S. Typhimurium Serotypes (41.4%), 15 were S. enteritidis (32.7%), 2 were S. typhi (4.4%). Salmonella isolates were sensitive to all of the tested antibiotics, but they slightly exhibited resistance to ampicillin and Cefotaxime. All examined Salmonella serovars in this study showed positive amplification for the invA, spv, and ViaB genes virulence genes. Conclusion: These results would greatly help in understanding the prevalence of virulence genes and antibiotic sensitivity among Salmonella serovars in Hamburger, Vegetable salads and Cream-filled pastries |
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کلیدواژههای انگلیسی مقاله |
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نویسندگان مقاله |
| Mansoureh Taghizadeh Food Quality Control Laboratory, Mazandaran University of Medical Sciences, Sari, Iran
| Behzad Javadian Amol Faculty of Paramedics, Mazandaran University of Medical Sciences, Sari, Iran
| Alireza Rafiei Department of Immunology, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences School of Medicine, Sari, Iran.
| Azadeh Taraghian Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
| Mahmood Moosazadeh Assistant professor, Health Sciences Research center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, Iran
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نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-931-2&slc_lang=en&sid=1 |
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کد مقاله (doi) |
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زبان مقاله منتشر شده |
en |
موضوعات مقاله منتشر شده |
میکروبیولوژی |
نوع مقاله منتشر شده |
پژوهشی |
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