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، جلد ۱۳، شماره ۱، صفحات ۲۷-۳۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Vitellogenin Gene Expression and Sex Steroid Levels as Biomarkers in Yellowfin Seabream (Acanthopagrus latus) Exposed to Bisphenol-A |
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| چکیده انگلیسی مقاله |
Background: The egg yolk precursor protein vitellogenin (VTG) has proven to be a useful biomarker, used to identify organisms exposed to estrogenic compounds. Methods: We investigated variations in the VTG gene expression pattern and plasma sex steroid hormones concentrations in the yellowfin Seabream, Acanthopagrus latus, (A. latus) by various doses of bisphenol-A (BPA) exposure for 7 and 14 days. We developed a quantitative real time polymerase chain reaction (RT-PCR) assay for the expression of VTG gene in A. latus. The dose-response pattern of VTG gene expression in A. latus exposed to various doses of BPA w::as char::acterized. In order to design RT-PCR primers specific to A. latus VTG, a partial sequence of the VTG gene was obtained. Results: The RT-PCR assay was effective in detecting increased VTG gene expression in A. latus exposed to BPA. It also demonstrated that the VTG expression was affected by BPA in a dose and time-dependent manner. Plasma testosterone (T) levels were decreased in the treated fish in comparison with those found in the control group, when they were exposed to 100 µg/g of BPA and 2 µg/g of E2. In contrast, the plasma levels of 17β-estradiol (E2) were significantly increased in a dose-dependent manner. Conclusion: The results suggest that VTG mRNA quantification can provide a sensitive and early signal in the detection of estrogens in marine wildlife. It also indicated that BPA could lead to an imbalance of sex steroid hormones with potentially harmful consequences on sexually immature male A. latus. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Ahmad Negintaji
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology. Khorramshahr, Iran.
| Alireza Safahieh
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology. Khorramshahr, Iran.
| Hosein Zolgharnein
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology. Khorramshahr, Iran.
| Soheila Matroodi
Department of Marine Biology, Faculty of Marine Sciences, Khorramshahr University of Marine Science and Technology. Khorramshahr, Iran.
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| نشانی اینترنتی |
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-577-1&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2737/article-2737-2049511.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
تخصصی |
| نوع مقاله منتشر شده |
کاربردی |
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