|
|
، جلد ۶، شماره ۱۸، صفحات ۶۹۱-۶۹۸
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Effect of Acute Toxicity of Cadmium in Mice Kidney Cells |
|
| چکیده انگلیسی مقاله |
Background: Cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. The present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium. Methods: Male rats (n=30), kept in standard conditions were used in this study. The animals were randomly divided into 2 groups (control and treatment). The treatment group was intraperitoneally injected with Cd (300µm/kg) at hours 0, 6, 12, 24, 48. Twenty four hours after the last injection, the rats were sacrificed and their kidneys were obtained. Then oxidative stress markers, malondialdehide (MDA), glutathione (GSH), and superoxide dismutase (SOD), were assayed in homogenized kidney for studying their cytotoxicity. For genotoxicity and DNA damage studies, Comet assay was run on isolated kidney cells. Data analysis was done by t-test and ANOVA using SPSS software version 15. Results: MDA and GSH concentrations in normal and Cd exposed kidney cells were 287.01±37.30nmol/g.pr and 15.61±3.89µmol/g.pr and 609.24±87.87nmol/g.pr and 28.52±5.22µmol/g.pr, respectively. In addition, SOD activity in normal and Cd exposed kidney cells were 77.75±4.12 and 218.91±5.40 U/mg.pr, respectively. Comet assay results (content comet length, tail length, and head diameter) showed DNA breakage in the treatment group that was stimulated by Cd which was not seen in the control group. Conclusion: The results demonstrated the genotoxicity effect of Cd on kidney cells as well as the ability of Cd to producing cytotoxicity. |
|
| کلیدواژه‌های انگلیسی مقاله |
Cadmium, Comet Assay, DNA Damage, Kidney, Oxidative Stress. |
|
| نویسندگان مقاله |
معصومه معصومی کریمی | Masoomeh Masoomi Karimi
Department of Immunology, Torbateheydariyeh University of Medical Sciences, Torbateheydariyeh, Iran.
مسلم جعفری ثانی | Moslem Jafari Sani
Department of Clinical Biochemistry, Torbateheydariyeh, University of Medical Sciences, Torbateheydariyeh, Iran.
علی زارعی محمودآبادی | Ali Zaree Mahmudabadi
Department of Molecular Biology Center, Baqiyatallah, University of Medical Sciences, Tehran, Iran.
اسما جعفری ثانی | Asma Jafari sani
Ms, Biology Research Center, Torbateheydariyeh, University of Medical Sciences, Torbateheydariyeh, Iran.
سید رضا خطیبی | Seyed Reza Khatibi
MD & Chief Student of MPH, Faculty of Torbatheydariyeh, University of Medical Sciences, Torbatheydariyeh, Iran.
|
|
| نشانی اینترنتی |
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-105&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2737/article-2737-2049771.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
تخصصی |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|