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Anatomical Sciences Journal، جلد ۷، شماره ۲۷، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In Vitro Development of Cattle Embryo as Affected By Glucose, Serum and EDTA |
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| چکیده انگلیسی مقاله |
Purpose: The aim of this study was to evaluate the effect of different modifications of sequential synthetic oviductal_fluid (SOF) culture system on developmental competence of in vitro matured/fertilized cattle embryos. Materials and Methods: Bovine oocytes were matured and fertilized in vitro and then presumptive zygotes were randomly cultured for up to 9 days in different modifications of SOF culture system to consider the effects of glucose, serum and EDTA on embryo development. Results: All the embryo culture systems were efficient to support bovine embryo development till blastocyst stage. There was no significant difference in the ratios of embryos; however, the ratios of blastocyst and also hatchability of embryos cultured in SOF C (51.3%, 43.0% and 83.8%, respectively) were significantly higher than those of all the other SOF groups. Furthermore, while glucose had a partial improving effect on embryo development, a significant decrease in embryo development beyond the morula stage was observed in embryos cultured in SOF system with initial supplementation of EDTA compared with all the other groups. Conclusion: It was concluded that appropriate modifications of SOF culture systems can result insignificantly great in vitro embryo development. |
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| کلیدواژه‌های انگلیسی مقاله |
In Vitro Embryo Development, Bovine Zygotes, Sof, Serum |
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| نویسندگان مقاله |
| Akbar Pirestani
| Seyed Morteza Hosseini
| Fariba Moulavi
| Mahdi Hajian
| Parvaneh Abedi
| Mohsen Forouzanfar
| Somayeh Ostad Hosseini
| Laleh Hosseini
| Hamid Ghasemzadeh Nava
| Parviz Tajik
| Mohammad Hasan Nasr Esfahani
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-180&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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