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Anatomical Sciences Journal، جلد ۵، شماره ۲۱، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Presence of Oxytocin Receptors in Embryonic Stem Cells and the Effects of Oxytocin Hormone on Mouse Embryonic Stem Cell-Derived Cardiomocyte Differentiation |
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| چکیده انگلیسی مقاله |
Purpose: This study sought to investigate the presence of oxytocin receptors and the possible biological role of oxytocin as an effective factor in the differentiationof embryonic stem cells into cardiomyocytes. Materials and Methods: Mouse ESCs were cultivated in hanging drops to form embryoid bodies (EBs). The EBs were then treated with and without oxytocin (experimental and control groups). Up to 30 days after plating, contraction and beating frequency were monitored and evaluated daily the characteristics of the ESC-derived cardiomyocytes were assessed by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results: In the experimental group, the percentage of the EBs with spontaneous contraction was significantly increased from 17th day onward. The beating cells of groups, stained positive with anti a-actinin, desmin, cardiactroponin I and connexin antibodies. Oxytocin receptors were detected on the ESCs and derived-differentiated cells. In addition, cardiac-specific genes such as cardiac a- and b-myosin heavy chain, myosin light chain-2v, and atrial natriuretic factor were also detected in the ESC-derived differentiated cells of both groups. In the experimental group, all the specific genes with the exception of α-myosin heavy chain, were more pronounced at the early stage of cardiomyocyte development.Conclusion: oxytocin has receptors on undifferentiated ESCs and derived differentiated cells, it can play role in development of cardiomyocytes during embryonic period. |
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| کلیدواژه‌های انگلیسی مقاله |
Mouse Embryonic Stem Cells, Cardiomyocyte Differentiation, Oxytocin, Oxytocin Receptor |
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| نویسندگان مقاله |
| Leili Hatami
| Mojtaba Rezazadeh Valoujerdi
| Seyed Javad Moula
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-257&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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