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Anatomical Sciences Journal، جلد ۵، شماره ۱۹، صفحات ۱۵۷-۱۶۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Effect of Different Doses of Staurosporine For Neuronal Differentiation |
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| چکیده انگلیسی مقاله |
Purpose: The present study was designed to investigate the effect of different doses of Staurosporine on differentiation of PC-12 cell line into differentiated neuronal cells. Materials and Methods: PC-12 cell line as a useful model system for neurobiological studies was maintained in RPMI 1640 supplemented with 10% FBS. Staurosporine were added to culture medium in different concentrations (110, 214, 316 and 1000nM), then morphology and apoptotic index were assessed by total neurite length estimation and TUNEL staining. Results: Data showed that total neurite length of PC-12 cells that were treated with 110, 214, 316 and 1000nM was 44±9.3, 153±15.0, 104±10.4 and 31±9.6μm respectively. Therefore PC-12 cells that were treated with 214nM Staurosporine had the greatest neurite length in compression with the other concentrations (P< 0.05). In addition apoptotic index in these concentrations was 3±0.7,4±0.8,8±1.0 and 10±1.1 respectively, Therefore PC-12 cells that were treated with 110 and 214nM Staurosporine had minimal apoptotic index (P< 0.05). Conclusion: Finally it can be concluded that 214nM concentration of Staurosporine is an optimum concentration for differentiation of neuronal cell lines to differentiated neuronal cells. |
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| کلیدواژه‌های انگلیسی مقاله |
Neuronal differentiation, Staurosporine, Apoptosis, PC-12 |
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| نویسندگان مقاله |
| Soheyl Sadri
| Maryam Davari Zanjani
| Mehri Azadbakht
Biology Department, Faculty Of Sciences, Razi University, Kermanshah, Iran.
| Ali Amini
| Iraj Amiri
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-178&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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