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Anatomical Sciences Journal، جلد ۳، شماره ۳، صفحات ۱۸۵-۱۹۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Molecular Cloning and Mutagenesis of Rat Glucocerebrosidase Gene |
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| چکیده انگلیسی مقاله |
Purpose: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it. Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced. Results: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing. Conclusion: The successful sequenced Gba cDNA compared with other researchers results. |
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| کلیدواژه‌های انگلیسی مقاله |
Glucocerebrosidase, Cloning, Pucbm21 in frame mutation |
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| نویسندگان مقاله |
| Homayoun Naderian
Anatomy Department, Medical Facalty, Kashan Medical Sciences University, Kashan, Iran.
| Bahram Kazemi
| Ahmad Hoseini
| Yoosef Sadeghi
| Antovan De Vries
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-109&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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