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Anatomical Sciences Journal، جلد ۳، شماره ۲، صفحات ۱۰۹-۱۱۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Effect of Β-Mercaptoethanolon Resumption of Meiosis, in Vitro Maturation and Development of Immature Mouse Oocyte Treated With Dehp (Di-(2-Ethylhexy) Phathalae) |
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| چکیده انگلیسی مقاله |
Purpose: The aim of this study was evaluated the effect of β -mercaptoethanol on resumption of meiosis, In vivo maturation of immature mouse oocytes treated with DEHP and embryo development. Materials and Methods: NMRI mouse strain (4-6 weeks) were treated daily with 50,µI DEHP Group 1: 121 immature oocytes were matured in MEM-α medium contain FCS 5%, hCG 7.5 IU/ml, rFsH 100mIU/ml, Group 2: 120 immature oocytes were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFsH 100mIU/ml and 100µm β-mercaptoethanol.Control group: 198 immature mouse oocytes recovered from nontreated mouse and were matured in MEMα medium contain FCS 5%, hCG 7.5IU/ml, rFSH 100mlU/ml. Fertilization and embryo development were done in T6 medium. Results: The resumption of meiosis in experimental groups (1&2) were (71%, 79%) respectively. However the resumption of meiosis in experimental groups were significantly different compared to control group (89%) (p< 0.05). The In vitro maturation in experimental groups (1,2) were (52%, 68%) respectively. However the in vitro maturation in one group was significantly different compared to control group (75%) (p=0.00) and second group was significantly higher than one experimental group (p< 0.01).The embryo number, after 24th insemination in experimental groups (1&2) were (52%, 70%) respectively However the embryo number in first group was significantly lower than the control group (74%) (P< 0.05) and second group was significantly higher than the first group (p< 0.05). Conclusion: The results of this study showed that the β-mercaptoethanol (100µM) inhibited the negative effect of DEHP on In vitro maturation and embryo development. The β-mercaptoethanol has improved the resumption of meiosis, In vito maturation and embryo development. |
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| کلیدواژه‌های انگلیسی مقاله |
Immature oocyte, In vitro maturation, Mouse, Β-mercaptoethanol, Dehp (Di-(2-ethylhexy) phathalae) |
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| نویسندگان مقاله |
| Hosein Imani
Embryology Department, Royan Institute, Tehran, Iran.
| Azam Dalman
| Hoori Sepehri
| Saeed Kazemi Ashtiani
| Fatemeh Hasani
| Mojtaba Reza Zadeh
| Abdol Hosein Shah Verdi
| Hosein Bahar Vand
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| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-104&slc_lang=fa&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
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