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Basic and Clinical Neuroscience، جلد ۱۳، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
A New and Simple Method for Spinal Cord Injury Induction in Mice |
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| چکیده انگلیسی مقاله |
Introduction: Spinal cord injury (SCI) is a devastating disease with poor clinical outcomes. Animal models provide great opportunities to expand our horizons in identifying SCI pathophysiological mechanisms and subsequently introducing effective treatment strategies. The present study precisely introduces a new murine contusion model. Methods: A simple, economical and reproducible novel instrument was designed which consists of various sections, including a body part, an immobilization piece and a bar-shaped weight. The injury was inflicted to the spinal cord using an eight-gram weight for 5, 10 or 15 minutes after laminectomy at the T9 level in male C57BL/6 mice. Motor function, cavity formation, cell injury and macrophage infiltration were evaluated 28 days' post injury. Results: The newly designed instrument minimized adverse spinal movement during injury induction. Moreover, no additional devices, such as a stereotaxic apparatus, was required to stabilize the animals during surgical procedure. Locomotor activity was deteriorated after injury. Furthermore, tissue damage and cell injury were exacerbated by increasing the duration of weight exertion. In addition, macrophage infiltration around the injured tissue was observed 28 days' post injury. Conclusion: This novel apparatus could induce a controllable SCI with a clear cavity formation in mice. No accessory elements are needed, besides the main equipment, and it can be used in future SCI studies. |
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| کلیدواژه‌های انگلیسی مقاله |
Spinal cord injury, Animal models, Mice |
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| نویسندگان مقاله |
| Zahra Zeraatpisheh
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
| Esmaeil Mirzaei
Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
| Mohammad Nami
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
| Hamed Alipour
Department of Tissue Engineering & Applied cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
| Somayeh Ghasemian
Genetic Laboratory, Shiraz Fertility Center, Shiraz, Iran
| Hassan Azari
Department of Neurosurgery, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611.
| Hadi Aligholi
Department of Neuroscience, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-35-3&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Behavioral Neuroscience |
| نوع مقاله منتشر شده |
Original |
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