|
|
Basic and Clinical Neuroscience، جلد ۱۲، شماره ۶، صفحات ۷۳۷-۷۴۴
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
The Repression of Matrix Metalloproteinases and Cytokine Secretion in Glioblastoma by Targeting K+ Channel |
|
| چکیده انگلیسی مقاله |
Introduction: Glioblastoma is an aggressive human brain malignancy with poorly understood pathogenesis. Voltage-gated potassium (Kv) channels and Matrix Metalloproteinases (MMPs) are highly expressed in malignant tumors and involved in the progression and metastasis of glioblastoma. This study aimed to determine whether a voltage-dependent potassium channel blocker could modulate astrocytes as a cell involved in the immunopathogenesis of glioblastoma. Methods: The cytotoxic effect of 4-Aminopyridine (4-AP) at different doses in the cell model of glioblastoma was measured by MTT assay. The ELISA technique and gelatin zymography were used to assess cytokine levels and MMP-9 after 4-AP treatment. Results: Cytotoxicity analysis data indicated that cell viability reduced by increasing 4-AP level and cell growth decreased gradually by removing 4-AP from the cell medium. 4-AP inhibits the secretion of IL-6 and IL-1 (P< 0.05). MMP9 activity significantly inhibits with increased 4-AP dose, compared to non-treated cells. Conclusion: The reduction of cell viability, IL-6 secretion, and MMP-9 activity in an in vitro model of glioblastoma might be assumed 4-AP as an agent for chemoprevention of cancer. |
|
| کلیدواژه‌های انگلیسی مقاله |
Glioblastoma, Interleukin-6, 4-Aminopyridine, Matrix metalloproteinases, Potassium channels, Voltage-gated |
|
| نویسندگان مقاله |
| Farshid Saadat
Department of Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
| Zohreh Zareighane
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
| Farnaz Safavifar
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
| Seyedeh Zohreh Jalali
Department of Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
| Azar Berahmeh
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
| Mohammad Reza Khorramizadeh
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
|
|
| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1693-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|