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Basic and Clinical Neuroscience، جلد ۱۲، شماره ۵، صفحات ۶۱۷-۶۲۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Neurosphere-Free Transdifferentiation of Rat Bone Marrow Stromal Stem Cells Into Retinal Cells and Retinal Pigment Epithelium |
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| چکیده انگلیسی مقاله |
Introduction: Neurosphere-free transdifferentiation of bone marrow stem cells into Retinal Pigment Epithelium (RPE) and Retinal Cells (RCs) in vitro could offer an exceptional opportunity to study cell replacement in degenerative eye diseases. Thus, a simple and efficient protocol for retinal cells production from transdifferentiation of rat BMSCs in the neurosphere-free state is reported. Methods: Extracted BMSCs from hooded pigment rats were exposed to a single-step protocol, including neurosphere-free, containing a cocktail medium that induced transdifferentiation into Retinal Pigment Epithelium (RPE) and retinal cells. Results: The results showed morphological differentiation changes in vitro. Also, the expressed retinal pigment epithelium and retinal cell markers, such as retinal orthodenticle homeobox 2 (23.45%), retinal pigment epithelium protein 65 (91.54%), cellular retinaldehyde-binding protein (91.21%), vascular endothelial growth factor (94.79%), rhodopsin (57.19%), glial fibrillary acidic protein (28.33%), and neurofilament 200 (24.55%). Conclusion: Overall, these findings showed that a protocol without using basic fibroblast growth factor, epidermal growth factor, and B-27 supplements could generate RPE and retinal cells in vitro. |
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| کلیدواژه‌های انگلیسی مقاله |
Neurosphere-free, Rat bone marrow stem cells, Retinal cells, Retinal pigment epithelium, Transdifferentiation |
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| نویسندگان مقاله |
| Hamid AboutalebKadkhodaeian
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
| Hamidreza Sameni
Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
| Ali Shahbazi
Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1055-3&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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