| چکیده انگلیسی مقاله |
Introduction: Multiple Sclerosis (MS) is the chronic inflammation of the Central Nervous System (CNS) and autoimmune disease. MS is most widely considered to be mediated by the activation of myelin-specific T CD4+ cells as well as TH1 and TH17 cells. TH17 cells are involved in the pathogenesis of MS in various manners. HIF-1α and RORC are required for the natural differentiation of TH17; they are essential transcription factors for the evolution of TH17 cells. Numerous studies indicated that Epigallocatechin Gallate (EGCG) presents immunomodulatory and anti-inflammatory effects. This study investigated the effects of EGCG on normoxic HIF-1α and RORC2 expression in PBMCs among MS patients. Methods: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from the whole blood of new cases of MS. The cells were cultured in the presence of a different concentration of EGCG (25, 50,100μM) for 18 and 48 hours. Next, HIF-1α and RORC2 level expressions were measured by Enzyme-Linked Immunosorbent Assay (ELISA) and Real-Time PCR, respectively. Results: The results showed that EGCG significantly decreased RORC2 gene expression. EGCG did not affect the level of HIF-1α. Conclusion: However, EGCG did not influence the level of HIF-1α. Our present data has led us to conclude that EGCG could be considered as an anti-inflammatory agent may serve as an achievable therapeutic agent for MS. |
| نویسندگان مقاله |
| Boshra Afshar
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Mazdak Ganjalikhani-Hakemi
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Zahra Khalifezadeh Esfahani
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Nahid Eskandari
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Vahid Shaygannajad
Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Fahimeh Hosseininasab
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
| Freshteh Alsahebfosoul
Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
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