|
|
Basic and Clinical Neuroscience، جلد ۱۱، شماره ۶، صفحات ۷۸۱-۷۹۴
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Diabetic Encephalopathy Affecting Mitochondria and Axonal Transport Proteins |
|
| چکیده انگلیسی مقاله |
Introduction: Diabetic encephalopathy is described as any cognitive and memory impairments associated with hippocampal degenerative changes, including the neurodegenerative process and decreased number of living cells. Mitochondrial diabetes (MD) appears following activation of mutant mitochondrial DNA and is a combination of diabetes and cognitive deficit. In this research, we showed the correlation of diabetic encephalopathy, dysfunctional mitochondria, and changes in the expression of axonal transport proteins (KIF5b, Dynein). Methods: Twenty-four male Wistar rats were divided into three groups: (n=8 in each group):1. Control + saline; 2. Diabetic, and 3. Diabetic + insulin. Before starting the experiments, the animals with blood sugar lower than 150 mg/dL entered the study. Diabetes induction was carried out by Intraperitoneal (IP) Streptozotocin (STZ) administration. Fasting Blood Sugar (FBS) and body weight was checked after the first week and at the end of the eighth week. Then, behavioral studies (elevated plus maze, Y-maze, and passive avoidance learning) were performed. After behavioral studies, blood samples were taken to measure serum insulin level and HgbA1c. Next, fresh hippocampal tissue was collected. Gene expression of motor proteins was assessed by real-time PCR and mitochondrial membrane potential by rhodamine123. Results: Our results showed the impairment of HgbA1c, serum insulin, FBS, and weight in the diabetic group (P< 0.05). Behavioral tests revealed different degrees of impairment in diabetic rats (P< 0.05). KIF5b mRNA expression increased in the hippocampus (P< 0.05) with no change in dynein gene expression. These changes were associated with abnormal mitochondrial membrane potential (P< 0.05). Conclusion: KIF5b mRNA up-regulation in hippocampal neurons of STZ-diabetic rats is a factor that can be involved in abnormal axonal transport and decreased MMP, leading to impairment of mitochondrial function. These manifestations showed mitochondrial dysfunction in diabetes and resulted in abnormal behavioral tests and diabetic encephalopathy. |
|
| کلیدواژه‌های انگلیسی مقاله |
Diabetes mellitus type 1, Mitochondrial encephalopathy, Axonal transport, Mitochondria, KIF5b protein, Dynein |
|
| نویسندگان مقاله |
| Maryam Eslami Gharaati
Department of Physiology, School of Medicine, Iran University of Medical Science, Tehran, Iran.
| Arezo Nahavandi
Department of Physiology, School of Medicine, Iran University of Medical Science, Tehran, Iran.
| Torandokht Baluchnejad Mojarad
Department of Physiology, School of Medicine, Iran University of Medical Science, Tehran, Iran.
| Mehrdad Roghani
Departmentof Physiology, Neurophysiology Research Center, Shahed University, Tehran, Iran.
|
|
| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-1657-1&slc_lang=en&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|