| چکیده انگلیسی مقاله |
Background: The effects of Pb-induced neurotoxicity have been largely established however the need for proper neuroprotective agents to mitigate the effects Pb-induced neurotoxicity remains vague. Aim: The study was aimed at investigating the neuroprotective effects of vitexin and C. cajan (extract) pre-treatment against Pb-induced neurotoxicity in Wistar rats. Materials and methods: Twenty-four male Wistar rats were randomly assigned into four groups (n=6). The control group was administered 0.5 ml/kg of distilled water. Pb group received, Pb acetate (200 mg/kg). Vitexin + Pb group, received Vitexin (50 mg/kg) an hour before Pb acetate (200 mg/kg). C. cajan + Pb group, received C. cajan (50 mg/kg) an hour before Pb acetate (200 mg/kg). All administration was within a duration of 28 days. Barnes maze test and NOR test were done to ascertain working memory, while the level of oxidative stress markers (MDA, SOD and GPx) were also tested. In addition, Prefrontal cortical sections were stained with H&E stain, while immunoreactivity of Iba1 and Nrf2 were examined. Results: There was a significant decline in working memory in the Pb group, but good working memory was maintained in vitexin and C. cajan pretreated groups. In vitexin and C. cajan pretreated groups, oxidative stress, neuronal damage and expression of Iba1 were significantly low when compared with Pb group. Nrf2 expression in Pb group was significantly low when compared with other groups. Conclusion: Results of this study have shown that pretreatment with vitexin and C.cajan offers neuroprotection against Pb toxicity via antioxidant and anti-inflammation actions. Although both vitexin and C. cajan extract showed neuroprotective abilities, vitexin exhibited better result. Background: The effects of Pb-induced neurotoxicity have been largely established however the need for proper neuroprotective agents to mitigate the effects Pb-induced neurotoxicity remains vague. Aim: The study was aimed at investigating the neuroprotective effects of vitexin and C. cajan (extract) pre-treatment against Pb-induced neurotoxicity in Wistar rats. Materials and methods: Twenty-four male Wistar rats were randomly assigned into four groups (n=6). The control group was administered 0.5 ml/kg of distilled water. Pb group received, Pb acetate (200 mg/kg). Vitexin + Pb group, received Vitexin (50 mg/kg) an hour before Pb acetate (200 mg/kg). C. cajan + Pb group, received C. cajan (50 mg/kg) an hour before Pb acetate (200 mg/kg). All administration was within a duration of 28 days. Barnes maze test and NOR test were done to ascertain working memory, while the level of oxidative stress markers (MDA, SOD and GPx) were also tested. In addition, Prefrontal cortical sections were stained with H&E stain, while immunoreactivity of Iba1 and Nrf2 were examined. Results: There was a significant decline in working memory in the Pb group, but good working memory was maintained in vitexin and C. cajan pretreated groups. In vitexin and C. cajan pretreated groups, oxidative stress, neuronal damage and expression of Iba1 were significantly low when compared with Pb group. Nrf2 expression in Pb group was significantly low when compared with other groups. Conclusion: Results of this study have shown that pretreatment with vitexin and C.cajan offers neuroprotection against Pb toxicity via antioxidant and anti-inflammation actions. Although both vitexin and C. cajan extract showed neuroprotective abilities, vitexin exhibited better result. |