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، جلد ۸، شماره ۲، صفحات ۱۵۵-۱۶۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Optimized Primary Culture and Subculture of Granulosa Cells |
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| چکیده انگلیسی مقاله |
Background: Primordial follicle includes an oocyte surrounded by a layer of somatic cells called Granulosa Cells (GCs). GCs, also known as nurse cells, are an important protective element for the growth and survival of oocytes. Oocytes, which lack some of the metabolic processes, require granulosa cells for their development. Objectives: This manuscript was provided to explain the protocol of GCs primary culture extracted from NMRI mice ovaries. Methods: For choosing the optimum protocol, we used two methods with different culture mediums to obtain more GCs and expedite the process. Hematoxylin and Eosin (H&E) staining and flow cytometry were used to analyze the type of extracted cells from ovaries. Besides, we evaluated the effect of crocin and DPP as two common natural products in Iran on the proliferation of these cells via MTT assay. Results: Second protocol method and alpha-MEM culture medium were chosen based on the results. Our findings from HE staining and flow cytometry proved the percentage of cultured GCs in the flask. Further, MTT assessment demonstrated that crocin at high doses had a toxic effect on granulosa cells, whereas date palm pollen (DPP) stimulated them to proliferation. Conclusion: Modifying this protocol is for the improvement of proliferation, coherence, and quality of GCs in primary culture and subculture. Regarding the effect of these two natural products on granulosa cells, we can mention the bilateral effect of crocin and DPP enhancement in proliferation. |
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| کلیدواژه‌های انگلیسی مقاله |
Cell culture techniques, Crocin, Date palm, Granulosa cells, Oocytes |
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| نویسندگان مقاله |
| Nadia Fallah
Department of Cellular and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
| Maryam Paktinat
Department of Cellular and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
| Milad Rasouli
Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
| Mohammad Nabiuni
Department of Cellular and Molecular Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
| Elaheh Amini
Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.
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| نشانی اینترنتی |
http://pbr.mazums.ac.ir/browse.php?a_code=A-10-1003-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بیولوژی سلولی- مولکولی |
| نوع مقاله منتشر شده |
پژوهشی |
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