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Basic and Clinical Neuroscience، جلد ۱۴، شماره ۴، صفحات ۴۵۳-۴۶۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Ferulago angulata Methanolic Extract Protects PC12 Cells Against Beta-amyloid-induced Toxicity |
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| چکیده انگلیسی مقاله |
Introduction: Alzheimer's disease (AD) is an age-dependent neurodegenerative disease. Beta-amyloid (Aβ)-induced neurotoxicity has a pivotal role in AD pathogenesis; therefore, the modulation of Aβ toxicity is the promising therapeutic approach to control the disease progression. Medicinal plants because of their multiple active ingredients are effective in complex diseases, such as AD. Therefore, several studies have studied medicinal plants to find an effective treatment for AD. Ferulago angulata is a medicinal plant with antioxidant and neuroprotective activity. The present study was done to assess the protective effect of the methanolic extract of Ferulago angulate on Aβ-induced toxicity and oxidative stress in PC12 cells. Methods: The methanolic extract of aerial parts of the plant was prepared by the maceration method. PC12 cells were cultured according to a standard protocol. PC12 cells were incubated for 24 hours with Aβ alone, and Aβ in combination with various concentrations of the F. angulata extract. Cell viability was determined by the methyl thiazole tetrazolium (MTT) assay. Also, reactive oxygen species (ROS) production and the activity of acetylcholine esterase (AChE), glutathione peroxidase (GPx), and caspase-3 enzymes were measured. Results:The extract dose-dependently protected PC12 cells against Aβ-induced cell death. Also, Aβ increased ROS production, AChE, and caspase-3 activity, and decreased the GPx activity, which all were ameliorated by F. angulata extract. Conclusion: F. angulata extract protects against Aβ-induced oxidative stress and apoptosis. These effects may be due to the antioxidant and anticholinesterase activity of the extract. It is recommended to assess F. angulata extract as an anti-AD agent. |
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| کلیدواژه‌های انگلیسی مقاله |
Beta-amyloid, Oxidative stress, Ferulago angulate, Alzheimer’s disease |
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| نویسندگان مقاله |
| Leila Hashemi
Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
| Maliheh Soodi
Institute for Natural Products and Medicinal Plants, Tarbiat Modares University, Tehran, Iran.
| Homa Hajimehdipoor
Department of Traditional Pharmacy, Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Abolfazl Dashti
Department of Forensic Toxicology, Legal Medicine Research Center, Iranian Legal Medicine Organization, Tehran, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-919-2&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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