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Immunoregulation، جلد ۴، شماره ۲، صفحات ۱۰۱-۱۰۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Recombinant Rabies Virus With a Multiple Cloning Site: A Platform Capable of Immunological Alterations |
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| چکیده انگلیسی مقاله |
Background: Rabies is fatal encephalitis, i.e., preventable by appropriate vaccination. Reverse genetics has proved promising for manipulating the rabies virus immunological characteristics. The insertion or deletion of a gene from the rabies genome could render specific functions to the rabies virus.
Materials and Methods: A multiple cloning site including 111 nucleotides long harboring 10 single-cut restriction sites have been designed. The designed fragment was cloned between the G and L genes of the rabies virus genome. The recombinant rabies virus was rescued, and its infectivity was confirmed in the BHK-21 cell line. The recombinant virus propagation was compared with the initial rabies virus strain. Statistical analysis was performed using GraphPad Prism.
Results: The cloning and localization of the multiple cloning site were verified by nucleotide sequencing. The recombinant virus properly propagated and rescued in the BHK-21 cell line. Comparing the recombinant virus with the initial rabies virus has shown that both viruses had similar functionality and propagation rate.
Conclusion: The recombinant virus obtained in the present study could facilitate further cloning experiments. Examples include constructing a marker virus, carrying green fluorescent protein to be used either in rabies immunity assays or tracking the virus infection in relevant tissues. |
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| کلیدواژههای انگلیسی مقاله |
Rabies virus,
Reverse genetics,
Virus engineering,
Molecular cloning |
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| نویسندگان مقاله |
Seyed Hamidreza Naghi Mousavi |
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
Alireza Gholami |
Viral Vaccines Production Unit, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran.
Mojtaba Jafarinia |
Department of Biology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
Mehdi Ajorloo |
Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
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| نشانی اینترنتی |
https://immunoreg.shahed.ac.ir/article_3608_07d4d2fd19a044e9b818a3ae7adf8082.pdf |
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| زبان مقاله منتشر شده |
en |
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