| چکیده انگلیسی مقاله |
Background: Polycystic ovary syndrome (PCOS) often is associated with low-quality oocytes. The aim of the present study was to investigate the effects of N-acetyl cysteine (NAC), melatonin and their combination on follicular fluid (FF) parameters, oocytes and embryo quality in PCOS patients.
Methods: A prospective randomized placebo-controlled pilot study on 60 Iranian women with PCOS undergoing intracytoplasmic sperm injection (ICSI) was designed. Women were divided into four groups (n=15 in each): (1) melatonin group, administered 3 mg/day melatonin at 22:00; (2) NAC group, administered 1800 mg day-1 NAC; (3) NAC+melatonin group, and (4) placebo group. Drugs were administered from the 5th day of previous cycle until the day of oocyte aspiration (40 days treatment in total). Hormonal status, sperm parameters, oocyte morphology, fertilization rate and embryo quality were assessed. Data were analyzed by one-way ANOVA, with significance set at P≤0.05.
Results: The number of immature and abnormal oocytes decreased significantly in the NAC (1.27±0.88 (P=0.006) and 2.1±1.1 (P=0.001), respectively) compared with placebo group (3.33±1.35 and 3.7±1.7, respectively), with a concomitant increase in the number of good embryos (Grade I) in the NAC (2.87±1.45, P=0.02) in comparison to placebo (1.53±0.83) group. Insulin and luteinizing hormone (LH) levels were significantly lower in the melatonin (7±2.4 and 1.5±0.5, respectively) and NAC (7.1±2.5 and 1.5±0.5, respectively) groups (P=0.01) compared with the placebo-treated group (9.5±2.9 and 1.8±0.6, respectively).
Conclusion: NAC improves oocyte and embryo quality, but combination of NAC and melatonin do not apply a significant positive effect. |
| نویسندگان مقاله |
| Sajed Khaledi
Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
| Maryam Najafpour Pitka
Department of Genetics, Tonekabon Islamic Azad University, Tonekabon, Iran.
| Zahra Sadat Hoseini
Department of biology, Faculty of genetics, Arsanjan Islamic Azad University, Arsanjan, Iran.
| Mehrdad Ghorbanlou
Department of Anatomical Science, Iran University of Medical Science, Tehran, Iran.
| Reza Shirazi
Department of Anatomy, School of Medical Sciences, Biomedical & Health, UNSW Sydney, Sydney, Australia.
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