|
|
Iranian Journal of Veterinary Medicine، جلد ۱۹، شماره ۳، صفحات ۵۰۳-۵۱۴
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
Physicochemical Parameters Affecting the In-vitro Toxins Production by Characterized Antibiotic-resistant Clostridium Perfringens Toxinotype B Isolates |
|
| چکیده انگلیسی مقاله |
Background: Alpha, beta and epsilon toxins of Clostridium perfringens toxintype B are the major potent toxins involved in enterotoxemia. To combat this condition, proper vaccination of animals is required with an effective vaccine.
Objectives: This research aimed to characterize the resistant C. perfringens type B isolates for in-vitro toxin production potential under the influence of various physicochemical parameters.
Methods: C. perfringens isolates were characterized based on biochemical tests, toxinotyping, and 16S rRNA typing. The indigenously characterized C. perfringens toxinotype B isolates (n=6) were subjected to antibiotic susceptibility profiling through the Kirby-Bauer method. The resistant C. perfringens type B isolates were subjected to toxin production optimization under physicochemical parameters (physical: Temperature, pH and time of incubation; chemical: Glucose, vitamin-mineral mixture, tween 80 and sodium salts at various concentration).
Results: The C. perfringens isolates were identified as toxinotype B. Isolates MW551887.1 and MW332247.1 produced higher hemolytic and cytotoxic units of toxins at a 0.2% glucose concentration in the broth after 24 hours at 37 ºC, respectively.
Alpha toxin, Beta toxin, Clostridium perfringens toxinotype B, Epsilon toxin, Glucose: To combat disease, controlling antibiotic resistance and ensuring proper vaccination of animals is crucial. These C. perfringens isolates may have commercial applications for toxoid vaccine production after further. Conclusion: To combat disease, control of antibiotic resistance and proper vaccination of animals is crucial. These C. perfringens isolates may have commercial application for toxoid vaccine production after further characterization and molecular testing of toxins. |
|
| کلیدواژه‌های انگلیسی مقاله |
Alpha Toxin,Beta toxin,Clostridium perfringens toxinotype B,Epsilon toxin,glucose |
|
| نویسندگان مقاله |
Madeeha Tariq |
Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Aftab Ahmad Anjum |
Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Wajeeha Tariq |
Institute of Biochemistry and Biotechnology, Faculty of Bioscience, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Tehreem Ali |
Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Rabia Manzoor |
Institute of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
|
|
| نشانی اینترنتی |
https://ijvm.ut.ac.ir/article_98912_4b15c800d46e401e5314ee40c466826c.pdf |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
|
| نوع مقاله منتشر شده |
|
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|