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Basic and Clinical Neuroscience، جلد ۱۶، شماره ۴، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Intranasal Administration of Autologous Conditioned Serum Attenuates Memory Impairment in Mice Model of Photothrombotic mPFC Ischemia |
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| چکیده انگلیسی مقاله |
Globally, stroke ranks as the second most prevalent cause of death, contributing significantly to worldwide mortality burdens, imposing a significant economic and emotional challenge on societies. This study designed to investigate the effect of autologous conditioned serum (ACS) on memory and associated molecular factors in a mouse model of photothrombotic ischemic stroke.
The photothrombotic model was used to induce medial prefrontal cortex (mPFC) ischemia. ACS were prepared by intracardiac puncture of C57BL/6 mice using special ACS syringes. After blood incubation, the sample was centrifuged, and the serum was analyzed with ELISA kits to quantified the levels of IL-1RA and IGF-I. The ischemic animals received 48 µl intranasal ACS in the periods of two times a day, once a day, or once every other day for one week. Behavioral tests, including the Lashley-III maze and social interaction test, were conducted following treatment administration. Additionally, the IGF-1, IL-1β, IL-1RA, levels, and phospho-tau/total-tau ratio were measured in the mPFC area by western blot. Histological analysis was performed to assess ischemic volume.
The results indicated that once-daily ACS administration significantly improved spatial memory in the Lashley-III maze and showed a notable enhancement in social memory as measured by the social interaction test. In terms of molecular analysis, ACS increased the levels of IGF-1 and IL-1RA, whilst decreasing the levels of IL-1β and p-tau/total-tau ratio.
In conclusion, post-stroke intranasal ACS administration enhances memory possibly by increasing the level of IGF-1 and attenuating inflammation through the inhibition of IL-1β signal by IL-1RA, and regulation of tau levels. |
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| کلیدواژه‌های انگلیسی مقاله |
Ischemic Stroke, Autologous conditioned serum, phosphorylated tau, interleukin-1 receptor antagonist, insulin like growth factor-1 |
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| نویسندگان مقاله |
| Sareh Kazmi
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Neda Yazdanfar
Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
| Fatemehsadat Seyedaghamiri
Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
| Alireza Pishgahi
Physical Medicine and Rehabilitation Specialist, Motahari General Hospital, Isfahan, Iran.
| Mehdi Yousefi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Tahereh Ghadiri
Department of Neuroscience, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
| Saeed Sadigh-Eteghad
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
| Mehdi Farhoudi
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-7486-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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