| چکیده انگلیسی مقاله |
Introduction: Mesenchymal stem cells (MSCs) are suitable candidates for the treatment of liver diseases. However, their low survival rate limits their efficacy following transplantation. This study aimed to evaluate the therapeutic potentials of H2O2-preconditioned umbilical cord-derived MSCs (UCMSCs) on acute liver failure (ALF) in mice. Methods: UCMSCs were pre-conditioned with different concentrations of H2O2. Cell viability was evaluated by WST-1 (water soluble tetrazolium) assay followed by exposure to lethal doses of H2O2. ALF was induced in NMRI mice using CCl4 and the cells therapy was performed using H2O2-preconditioned and normal UCMSCs. After 24, 48, and 72 hours, regenerative potentials of different UCMSCs groups were evaluated compared to sham group (that receive no MSCs) using biochemical and histological methods. Results: Lower liver enzymes was significantly evident in mice transplanted with H2O2-preconditioned UCMSCs compared with the other groups. Interestingly, histological results revealed a significant improvement in liver regeneration in these mice. Conclusion: Preconditioning of UCMSCs with H2O2 not only enhances their survival but also increases the efficacy of MSCs-based cell therapy in acute liver failure. |
| نویسندگان مقاله |
فاطمه نصیری | fatemeh nasiri
department of biochemistry, high institute for research and education in transfusion medicine, tehran, iran.
بهروز جوهری | behrouz johari
department of biotechnology, pasteur institute of iran, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
فاطمه امیری | fatemeh amiri
department of biochemistry, high institute for research and education in transfusion medicine, tehran, iran.
سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)
مهریار حبیبی رود کنار | mehryar habibi roud kenar
department of biochemistry, high institute for research and education in transfusion medicine, tehran, iran.
صدیقه مولایی | sedigheh molaei
department of biochemistry, school of medicine, qom university of medical sciences, qom, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی قم (Qom university of medical sciences)
مرضیه بهادری | marzieh bahadori
department of biochemistry, high institute for research and education in transfusion medicine, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی قم (Qom university of medical sciences)
مرجان موحد | marjan movahed
اباذر یاری | abazar yari
department of anatomy, school of medicine, alborz university of medical sciences, karaj, iran.
محمد علی جلیلی | mohamad ali jalili
department of biochemistry, high institute for research and education in transfusion medicine, tehran, iran.
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