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Basic and Clinical Neuroscience، جلد ۸، شماره ۵، صفحات ۳۸۷-۳۹۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Transdifferentiation of Human Dental Pulp Stem Cells Into Oligoprogenitor Cells |
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| چکیده انگلیسی مقاله |
Introduction: The nerve fibers in central nervous system are surrounded by myelin sheet which is formed by oligodendrocytes. Cell therapy based on oligodendrocytes and their precursors transplantation can hold a promising alternative treatment for myelin sheet repair in demyelinating diseases. Methods: Human Dental Pulp Stem Cells (hDPSCs) are noninvasive, autologous and easy available source with multipotency characteristics, so they are in focus of interest in regenerative medicine. In the present study, hDPSCs were differentiated into oligoprogenitor using glial induction media, containing Retinoic Acid (RA), basic Fibroblast Growth Factor (bFGF), Platelet-Derived Growth Factor (PDGF), N2 and B27. The differentiated Oligoprogenitor Cells (OPCs) were evaluated for nestin, Olig2, NG2 and O4 using immunocytochemistry. Also, the expression of nestin, Olig2 and PDGFR-α gens (neuroprogenitor and oligoprogenitor markers) were investigated via RT-PCR technique. Results: The results indicate that glial differentiation medium induces the generation of oligoprogenitor cells as revealed via exhibition of specific glial markers, including Olig2, NG2 and O4. The expersion of nestin gene (neuroprogenitor marker) and Olig2 and PDGFR-α genes (oligoprogentor markers) were detected in treated hDPSCs at the end of the induction stage. Conclusion: hDPSCs can be induced to transdifferentiate into oligoprogenitor cells and respond to the routinely applied regents for glial differentiation of mesanchymal stem cells. These data suggest the hDPSCs as a valuable source for cell therapy in neurodegenerative diseases. |
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| نویسندگان مقاله |
اردشیر معیری | ardeshir moayeri
department of anatomy, faculty of medicine, ilam university of medical sciences, ilam, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)
مریم نظم بجنوردی | maryam nazm bojnordi
department of anatomy, faculty of medical sciences, mazandaran university of medical sciences,sari, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
سارا هراتی زاده | sara haratizadeh
department of anatomy amp; cell biology, faculty of medicine, mazandaran university of medical sciences, sari, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
امیر اسماعیل نژاد مقدم | amir esmaeilnejad moghadam
department of anatomy amp; cell biology, faculty of medicine, mazandaran university of medical sciences, sari, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
رفیعه علیزاده | rafieh alizadeh
ent and head amp; neck research center and department, hazrat rasoul akram hospital, iran university of medical sciences iums , tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
هاتف قاسمی حمیدآبادی | hatef ghasemi hamidabadi
department of anatomy, faculty of medical sciences, mazandaran university of medical sciences, sari, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-973-1&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/198/article-198-432169.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Cellular and molecular Neuroscience |
| نوع مقاله منتشر شده |
Original |
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