Basic and Clinical Neuroscience، جلد ۸، شماره ۶، صفحات ۰-۰

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عنوان انگلیسی Protective Role of Hypothermia Against Heat Stress in Differentiated and Undifferentiated Human Neural Precursor Cells: A Differential Approach for the Treatment of Traumatic Brain Injury
چکیده انگلیسی مقاله Introduction: The present study aimed to explore protective mechanisms of hypothermia against mild cold and heat stress on highly proliferative homogeneous human Neural Precursor Cells (NPCs) derived from Subventricular Zone (SVZ) of human fetal brain.  Methods: CD133+ve enriched undifferentiated and differentiated human NPCs were exposed to heat stress at 42°C. Then, Western-blot quantification was performed using Hsp-70 (70 kilodalton heat shock proteins) recombinant protein. Finally, changes in pluripotency and Hsp-70 expression were measured using immunofluorescence staining and RT-qPCR (Quantitative reverse transcription PCR) analysis, respectively.  Results: Heat stress resulted in abnormal neurospheres development. The apoptosis rate was enhanced during long-term in vitro culture of neurospheres. Neurogenic differentiation reduced and showed aberrent phenotypes during heat stress. After hypothermia treatment significant improvement in neurospheres and neuronal cell morphology was observed.  Conclusion: Mild-hypothermia treatment induces attenuated heat shock response against heat stress resulting in induced HSP-70 expression that significantly improves structure and function of both undifferentiated human NPCs and differentiated neurons.
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نویسندگان مقاله sandeep کومار vishwakarma | sandeep kumar vishwakarma
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


avinash بردیا | avinash bardia
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


nusrath فاطیما | nusrath fathima
department of genetics, faculty of science, osmania university, hyderabad, india.


lakkireddy chandrakala | lakkireddy chandrakala
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


سید rahamathulla | syed rahamathulla
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


nagarapu raju | nagarapu raju
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


gunda srinivas | gunda srinivas
centre for cellular and molecular biology, hyderabad, india.


avinash راژ | avinash raj
centre for cellular and molecular biology, hyderabad, india.


annamaneni sandhya | annamaneni sandhya
department of genetics, faculty of science, osmania university, hyderabad, india.


vishnupriya satti | vishnupriya satti
department of genetics, faculty of science, osmania university, hyderabad, india.


santosh کومار tiwari | santosh kumar tiwari
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


سید امیر basha paspala | syed ameer basha paspala
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.


aleem احمد خان | aleem ahmed khan
central laboratory for stem cell research and translational medicine, centre for liver research and diagnostics, deccan college of medical sciences, hyderabad, india.



نشانی اینترنتی http://bcn.iums.ac.ir/browse.php?a_code=A-10-786-1&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular and molecular Neuroscience
نوع مقاله منتشر شده Original
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