| چکیده انگلیسی مقاله |
Introduction: Natural biomaterials and growth factors are key factors in tissue engineering. The objective of the present study was to evaluate transforming growth factor beta 1 (TGF-β1) and curcumin on proliferation and differentiation of nasal-derived chondrocyte seeded on the fibrin glue scaffold. Methods: Chondrocytes were isolated from nasal samples. Nasal-derived chondrocytes were seeded on fibrin glue at chondrogenic induction medium for 2 weeks. In this study, the effects of various concentrations of curcumin and TGF-β1 on the survival and proliferation of chondrocytes seeded on fibrin biomaterial were assessed by MTT assays. Also, chondrocytespecific gene expression was assessed by real-time polymerase chain reaction (PCR). Results: There were significant differences among the group treated with curcumin 10 μg compared to other groups with regard to cell viability. Also, gene expression of collagen type II, aggrecan, and SOX9 in the chondrocytes seeded on fibrin biomaterial containing the growth factor TGF-β1 significantly differed from those of curcumin and control group. Conclusion: Our results indicate that TGF-β1 and natural biomaterial of curcumin can be used effectively in chondrogenic viability and differentiation of nasal-derived chondrocyte. |
| نویسندگان مقاله |
حسین قانع پور | hossein ghanepour
babol medical science university, babol, iran.
محمد مهدی زاده | mohammad mehdizadeh
babol medical science university, babol, iran.
ابراهیم ذبیحی | ebrahim zabihi
babol medical science university, babol, iran.
مهدیه غیاثی | mahdieh ghiasi
stem cell laboratory, the academic center for education, culture and research, qom branch, qom, iran.
محسن شیخ حسن | mohsen sheykhhasan
stem cell laboratory, the academic center for education, culture and research, qom branch, qom, iran.
میترا نرماشیری | mitra narmashiri
department of oral and maxillofacial surgery, school of dentistry, babol university of medical sciences, babol, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
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