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Anatomical Sciences Journal، جلد ۱۲، شماره ۱، صفحات ۳-۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Stable Transfection of pEGFP-N1-MOG Plasmid to Utilize in Multiple Sclerosis Gene Therapy |
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| چکیده انگلیسی مقاله |
Introduction: Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system. Methods: In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells. Results: Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa. Conclusion: Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis. |
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| کلیدواژه‌های انگلیسی مقاله |
MOG, Multiple sclerosis, Gene therapy |
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| نویسندگان مقاله |
محمد حسن کریم فر | mohammad hassan karimfar
organization
محسن noorozian | mohsen noorozian
organization
رضا ماستری فراهانی | reza mastery farahani
organization
مریم سادات خرمگاه | maryam sadat khoramgah
organization
هادی عظیمی | hadi azimi
organization
علی اصغر keramatinia | ali asghar keramatinia
organization
نوید احمدی روزبهانی | navid ahmadi rozbahani
organization
ایاد بهادری منفرد | ayad bahadori monfared
organization
حجت اله عباس زاده | hojjat allah abbaszadeh
department of biology and anatomical sciences, shahid beheshti university of medical sciences and health services, tehran, iran.
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
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| نشانی اینترنتی |
http://www.anatomyjournal.ir/browse.php?a_code=A-10-87-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
1 |
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