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Basic and Clinical Neuroscience، جلد ۶، شماره ۳، صفحات ۱۷۱-۱۷۸
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| عنوان فارسی |
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| عنوان انگلیسی |
Involvement of Mu Opioid Receptor Signaling in The Protective Effect of Opioid against 6-Hydroxydopamine-Induced SH-SY5Y Human Neuroblastoma Cells Apoptosis |
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| چکیده انگلیسی مقاله |
Introduction: The neuroprotective role of opioid morphine against 6-hydroxydopamineinduced cell death has been demonstrated. However, the exact mechanism(s) underlying such neuroprotection, especially the role of subtype receptors, has not yet been fully clarified. Methods: Here, we investigated the effects of different opioid agonists on 6-OHDA-induced neurotoxicity in human neuroblastoma SH-SY5Y cell line as an in vitro model of Parkinson’s disease. Cell damage was induced by 150 μM 6-OHDA and the cells viability was examined by MTT assay. Intracellular calcium, reactive oxygen species and mitochondrial membrane potential were assessed by fluorescence spectrophotometry method. Immunoblot technique was used to evaluate cytochrome-c and activated caspase-3 as biochemical markers of apoptosis induction. Results: The data showed that 6-OHDA caused significant cell damage, loss of mitochondrial membrane potential and increase in intracellular reactive oxygen species and calcium levels as well as activated caspase-3 and cytochrome-c release. Incubation of SH-SY5Y cells with μ-opioid agonists, morphine and DAMGO, but not with δ-opioid agonist, DADLE, elicited protective effect and reduced biochemical markers of cell damage and death. Discussion: The results suggest that μ-opioid receptors signaling participate in the opioid neuroprotective effects against 6-OHDA-induced neurotoxicity. |
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| نویسندگان مقاله |
شهرزاد افتخار واقفی | shahrzad eftekhar vaghefi
shahid bahonar university of kerman. kerman, iran
سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)
سعید اسماعیلی ماهانی | saeed esmaeili mahani
department of biology, faculty of sciences, shahid bahonar university of kerman, kerman, iran
سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)
لیلا الیاسی | leila elyasi
neuroscience research center, institute of neuropharmacology, kerman university of medical sciences. kerman, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)
مهدی عباس نژاد | mehdi abbasnejad
shahid bahonar university of kerman. kerman, iran
سازمان اصلی تایید شده: دانشگاه شهید باهنر (Shahid bahonar university)
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| نشانی اینترنتی |
http://bcn.iums.ac.ir/browse.php?a_code=A-10-668-1&slc_lang=en&sid=en |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Behavioral Neuroscience |
| نوع مقاله منتشر شده |
Original |
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