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Iranian Journal of Veterinary Medicine، جلد ۹، شماره ۲، صفحات ۱۰۹-۱۱۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Detection of cytolethal distending toxin (cdt) Genes in Campylobacter jejuni and Campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of Iran |
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| چکیده انگلیسی مقاله |
Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. Sharing of the cytolethal distending toxin (cdt) genes in Campylobacter is common and is considered species specific. OBJECTIVES: In this study we focused on detecting the presence of cdt gene in C. jejuni and C. coli isolated from broilers, turkeys and quails of Iran. METHODS: Cecal samples were randomly collected from 240 broiler chickens, 100 meat type turkeys and 100 quails after slaughtering. We used PCR as a method for detecting cdt genes. RESULTS: In broilers, 93% of 58 C. jejuni positive samples possessed cdt gene and in all cases the three different subunits of cdt genes were present. However, only 56% of 14 C. coli isolates in broilers had contained cdt genes, while one fourth having all three subunits present. In turkeys, around 65% of 34 C. jejuni positive samples had cdt gene present with 38% possessing all three subunits of cdt genes. But all 5 C. coli isolates had all three subunits cdt gene. In quails, 67% of 30 C. jejuni positive samples were identified by cdt gene, 20% of those possessed all three gene subunits. On the other hand, all 28 C. coli isolates of quails had cdt gene present while 36% of those held all three gene subunits. CONCLUSIONS: Our data is indicating the isolation, culture and cdt PCR amplification approaches in this study seemed to be efficient. However, the presence of different variation of Campylobacter cdt gene types in our sample isolates signifies the necessity of further functional gene studies to elucidate which gene type combinations result in encoding effective toxins. |
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| کلیدواژه‌های انگلیسی مقاله |
broilers,turkeys,quails,cdt gene,Campylobacter jejuni |
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| نویسندگان مقاله |
روجا شجاعی کاوان | shojaei kavan
department of avian diseases, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
محمد حسن زاده |
department of avian diseases, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
محمد حسن بزرگمهری فرد | mohammad hassan bozorgmehri fard
department of avian diseases, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
سید علی پوربخش | seyed ali
razi vaccine and serum research institute, karaj, iran
سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)
افشین آخوندزاده بستی | akhondzadeh basti
department of food hygiene and quality control, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
عباس برین |
department of clinical pathology, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
ایرج اشرفی |
department of microbiology, faculty of veterinary medicine, university of tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
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| نشانی اینترنتی |
http://ijvm.ut.ac.ir/article_54009_efc91e44085a9ec8dd3d6d1884b86de3.pdf |
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| زبان مقاله منتشر شده |
en |
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