| چکیده انگلیسی مقاله |
BACKGROUND: Stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal Mesenchymal Stem Cells (MSCs) have beencarried out. On the other hand, Cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. OBJECTIVES: The aim of this study was toisolate, characterize, and transduce cBM-MSCs. METHODS:Canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)were isolated from bone marrow of dogs andcharacterized based on morphology, differentiation capacities,and surface marker expressions. For the first time, we labeledcBM-MSCs by GFP-encoding lentiviral vector to track them.RESULTS: cBM-MSCs were successfully isolated and proliferated.Morphologically, these cells were similar to otherMSCs from other sources and species and were able todifferentiate into osteocytes and adipocytes. cBM-MSCsexpressed surface marker CD44 but were not able to expressCD34. Approximately, 70% of cells efficaciously expressedGFPafter labeling; CONCLUSIONS:We found that GFP labelingis an easy and effective technique to track transplanted cBMMSCs.Our results also provide fundamental information aboutcanine BM-MSCs in order to use in veterinary medicine. |
| نویسندگان مقاله |
مهدیه رضایی |
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
شهرام جمشیدی |
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
آنا صفارپور |
department of periodontology, international campus school of dentistry, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مهدی عاشوری |
department of oral and maxillofacial pathology, faculty of dentistry, shahed university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)
داود شریفی |
department of surgery and radiology, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
حامد zamankhan ملایری | zamankhan malayeri
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
naqa تمیمی |
department of internal medicine, faculty of veterinary medicine, university of tehran, tehran, iran
سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)
امیررضا رکن | amir reza
dental implant research center and department of periodontics, school of dentistry, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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