| چکیده انگلیسی مقاله |
Background: Due to the lack of efficient anti-HIV vaccine, anti-HIV pharmaceuticals play an important role in controlling HIV infection. Also significant rise in drug resistance and drug toxicity has caused increased interest in finding new anti-HIV agents. In this study, a nano-sized version of lamivudine based on PEGylated chitosan was synthesized. Materials and Methods: In this research, nanoparticles of chitosan were efficiently PEGylated for increasing their stability in water and then the anti-HIV drug, lamivudine, was loaded on these PEGylated nanoparticles. After purification and lyophilization of new synthesized nanoparticle, the raw materials and final product were sampled and FTIR, HNMR and CHN analyses were done. Results: Results of HNMR spectroscopy showed that chitosan nanoparticle was successfully PEGylated. HNMR data confirmed FTIR results and indicated that lamivudine was conjugated on chitosan nanoparticle. In addition, CHN analysis data also confirmed both HNMR and FTIR data, and demonstrated that a high yield of chitosan nanoparticle PEGylation (approximately 97%) was done and illustrated a high capacity of lamivudine conjugation on nano-sized PEGylated chitosan (30% W/W chitosan). Conclusion: In this study, lamivudine drug was successfully synthesized, based on PEGylated chitosan nanoparticle. |
| نویسندگان مقاله |
حسین حیدری | Hosein Heydari
1- Department of Biology, Islamic Azad University, Tehran, Iran
گروه زیست شناسی، دانشگاه آزاد اسلامی واحد علوم تحقیقات، تهران، ایران
مهدی شفیعی اردستانی | Mehdi Shafiee Ardestani
2- Assistant Professor, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
رضوان ذبیح الهی | Rezvan Zabihollahi
3- General Practitioner, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
سید مهدی سادات | Seyed Mehdi Sadat
2- Assistant Professor, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
شیوا ایرانی | Shiva Irani
4- Assistant Professor, Department of Biology, Islamic Azad University, Tehran, Iran
گروه زیست شناسی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران
سید نظام الدین حسینی | Seyed Nezamedin Hoseini
5- Assistant Professor, Department of Research and Production, Pasteur Institute, Tehran, Iran
بخش تولید فرآوردههای نو ترکیب، واحد تولیدی و تحقیقاتی انستیتو پاستور ایران، تهران، ایران
صفیه امینی | Safieh Amini
6- Associate Professor, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
سید داور سیادت | Seyed Davar Syadat
7- Associate Professor, Department of Bacteriology, Pasteur Institute, Tehran, Iran
بخش باکتری شناسی، انستیتو پاستور ایران، تهران، ایران
محمد صادق خسروی | Mohammad Sadegh Khosravi
8- Technician, Department of Rabidity, Pasteur Institute, Tehran, Iran
دکترای دام پزشکی، بخش هاری، انستیتو پاستور ایران، تهران، ایران
علیرضا عزیزی سراجی | Alireza Azizi Saraji
9- Student of Microbiology M.Sc, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
پونه رحیمی | Pouneh Rahimi
2- Assistant Professor, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
محمدرضا آقاصادقی | Mohammad Reza Aghasadeghi
6- Associate Professor, Department of Hepatitis and AIDS, Pasteur Institute, Tehran, Iran
بخش هپاتیت و ایدز، انستیتو پاستور ایران، تهران، ایران
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