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، جلد ۱، شماره ۲، صفحات ۶-۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Heavy Metal Induced Cell Necrosis: Involves Apoptosis Death Signals Initiated by Mitochondrial Injury |
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| چکیده انگلیسی مقاله |
Introduction: Severe industrial diseases result from the hepatic accumulation of mercury, cadmium or chromium in humans and on the other hand cadmium and dichromate and mercuric salts may induce lung or kidney cancer. Acute or chronic CdCl2, HgCl2 or dichromate administration induces hepatic and nephrotoxicity in rodents. Oxidative stress is often cited as a possible cause of metal induced cell death but the death signaling pathways involved have not yet been well investigated. Method and Materials: To search for death signaling mechanisms we used accelerated cytotoxicity mechanism screening techniques (ACMS) on isolated rat hepatocytes as our cellular model. Results: Adding the CdC12, HgC12 or K2Cr2O7 to isolated hepatocytes caused a rapid increase in reactive oxygen species ("ROS") formation and a decline in mitochondrial membrane potential. Then lipid per-oxidation and cell-lysis ensued. Cytotoxicity was prevented by "ROS" scavengers and various inhibitors of the mitochondrial permeability transition (MPT) e.g. cyclosporin A, carnitine or trifluoperazine. Antioxidants prevented hepatocyte lysis induced by CdC12 , K2Cr207 but not HgC12. Conclusion: Hepatocyte lysis was also prevented by various apoptosis inhibitors e.g. cycloheximide, dactinomycin and a tetrapeptide caspase 3 inhibitor which suggested that metal induced hepatocyte lysis involves apoptotic death signals initiated by MPT and "ROS". |
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| کلیدواژههای انگلیسی مقاله |
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| نویسندگان مقاله |
جلال پوراحمد | Jalal Pourahmad
Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Beheshti Medical University, Tehran, Iran.
فرزانه جوکار | Farzaneh Jokar
Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Beheshti Medical University, Tehran, Iran.
مریم ربیعی | Mariam Rabiei
Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shahid Beheshti Medical University, Tehran, Iran.
| Peter J.O Brien
Faculty of Pharmacy, University of Toronto, Toronto, Canada
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| نشانی اینترنتی |
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-6&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2737/article-2737-2049871.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
عمومی |
| نوع مقاله منتشر شده |
پژوهشی |
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