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، جلد ۷، شماره ۳، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Simple and Rapid Detection of Burkholderia and Variolla using Multiplex-PCR |
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| چکیده انگلیسی مقاله |
Background: Todays, one of the most important problems in detection of human pathogens, is lack of positive control. The idea of using hybrid vectors, containing genes of different pathogens, can overcome this limitation. We can design specific primers for each region and use the hybrid vector as positive control sample in PCR. In this research we designed a hybrid vector and relevant primers for detection of Variolla and Burkholderia. Materials and methods: In this study 16srRNA and HA genes were chosen to be located on the vector, to represent of Burkholderia and Variolla, in respectively. The sequence of these genes obtained from NCBI in FASTA format and aligned in BioEdit software for finding conserve region of each gene, then some purposeful changes were applied in the sequence of each gene and the sequences were placed next to each other and the construct was designed. Specific primers designed for each region using Oligo7, BioEdit, GeneRunner softwares, Oligo analyzer website and NCBI database. Finally, the construct cloned in PUC57 in SnapGene and PCRsimulated on hybrid vector using designed primers. Results: Analysis confirmed that conserved region for each gene is located on hybrid vector for each pathogen, and simulation of PCR proved the accuracy of designed primers. Conclusion: Hybrid vectors design contain similar sequence of pathogens genome but they are none-pathogenic. We can use these hybrid vectors as positive control, without any concern. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Mona Simkhah
MalekAshtar University of Technology, Tehran, Iran
| Mohammad Javad Dehghan Esmatabadi
MalekAshtar University of Technology, Tehran, Iran
| Mehdi Zeinoddini
MalekAshtar University of Technology, Tehran, Iran
| Nafiseh Pourmahdi
MalekAshtar University of Technology, Tehran, Iran
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1111-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
میکروبیولوژی |
| نوع مقاله منتشر شده |
پژوهشی |
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