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، جلد ۶، شماره ۱، صفحات ۴۱-۵۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Evaluation of Relationship between Single-nucleotide Polymorphism in TNF-gene Promoter and Susceptibility to Atherosclerosis in Fatemeh Zahra Hospital |
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| چکیده انگلیسی مقاله |
Background: Tumor necrosis factor-alpha (TNF-α) is a cytokine of proinflmmatory that elicits a polyvalent initial response of inflammatory cells in coronary atherosclerosis. Polymorphism and susceptibility to atherosclerosis may be related to the TNF-α gene promoter. The aim of this study was to investigate single nucleotide polymorphisms of the TNF-α gene promoter at two sites in patients with atherosclerosis referred to the Fatemeh al-Zahra Hospital, Sari City. Methods: This study was a case control study which involved 120 patients (>50% stenosis) and 120 healthy individuals (< 10% stenosis). Genomic DNA was extracted with the phenol-chloroform method from white blood cells. Genotypes and TNF-α gene polymorphisms were analyzed using RFLP-PCR. Genotype frequency analysis, Hardy-Weinberg equilibrium test, and chi-square analysis have been conducted using SPSS software, version 22. Results: Genotype frequencies of GA, GG, and AA at position –308 of the TNF-α gene promoter in patients were 12.5%, 75%, and 12.5%, respectively. The respective values in healthy subjects were 7.5%, 21.7%, and 70.8%. Allele A to G polymorphism increased the risk of disease by 12.716%. The genotype frequencies of the AC, CC, and AA at position –863 of the TNF-α gene promoter in patients were 3.3%, 69.2%, and 27.5%, respectively. The respective values in healthy individuals were 2.5%, 11.7%, and 85.8%. Allele A to C polymorphism increased the risk of the disease by 16.373%. The difference in the risk of atherosclerosis was significant (P < 0.05). Conclusion: Mutations in TNF-α gene promoter could increase susceptibility to atherosclerosis. Determination of the genotypes of the individuals in these regions can help identify patients at risk for this disease. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Somayyeh Nouri
Student of Toxicology of Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, University Tehran –Iran(IAUPS)
| Zahra Mousavi
Assistant Prof, Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran –Iran (IAUPS)
| Soheil Azizi
Associated prof. Department of Laboratory Medicine, Faculty of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari-Iran
| Babak Bagheri
Associated prof.Cardiology research center,Fatemeh Zahra hospital,Mazandaran university of medical sciences, Sari Iran
| Ramin Ataee
Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari-Iran.
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-925-1&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201166.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
قلب و عروق |
| نوع مقاله منتشر شده |
پژوهشی |
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