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، جلد ۵، شماره ۳، صفحات ۳۷-۴۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Molecular Detection of Zonula Occludens Toxin (zot) Genes in Vibrio Cholerae O1 using PCR |
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| چکیده انگلیسی مقاله |
Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplification was carried out by the zot gene-specific primers. Then, PCR products were investigated by electrophoresis on 1.2% agarose gel stained by ethidium bromide. Also, the specificity of primers was measured using bacterial samples other than V. cholerae, such as enterotoxigenic Escherichia coli )ETEC(, Salmonela. typhi and Aeromonas hydrophila. The sensitivity of the PCR reaction was also evaluated using serial dilutions of V. cholerae O1 concentration (cfu/ml). Results: The data showed that the designed primers specificity for zot gene was successful and the sensitivity of this method was determined about 142 cfu/ml. Conclusion: In conclusion, this molecular detection can be used as a simple diagnostic kit in clinical laboratories for identification of V. cholerae. |
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| کلیدواژه‌های انگلیسی مقاله |
Vibrio cholerae, zot, PCR, Diagnostic. |
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| نویسندگان مقاله |
| Seyedeh Mahnaz Mousavi
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
| Mehdi Zeinoddini
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
| Azadeh Azizi
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
| AliReza Saeedinia
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
| Arina Monazah
Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-903-2&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201181.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
زیست شناسی مولکولی |
| نوع مقاله منتشر شده |
پژوهشی |
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