| چکیده انگلیسی مقاله |
Background: Diabetes mellitus (DM) is characterized by the presence of hyperglycemia. It has been documented that oxidative stress and reactive oxygen species (ROS) production have a key role in the pathogenesis of diabetes and its complications. Neutrophils as a part of immune system produce ROS, neutrophils function might be altered in diabetes. Lamium album is known to have antioxidant, and free radical scavenging actions. The aim of the present study was to evaluate the potential effect of L. album on mitochondrial ROS production from circulating neutrophils in diabetic rats. Materials and Methods: Twenty-one male Wistar rats were randomly divided into three groups: normal control rats receiving daily saline; diabetic control rats receiving daily saline; and diabetic rats treated daily with hydroalcoholic extract of L. album (100 mg/kg) for 28 days. On the 28thday of treatment, whole blood samples were obtained and mitochondrial ROS of neutrophils were measured by dihydrorhodamine (DHR) flow cytometric method. Also, fasting blood sugar (FBS) was measured. Results: Mitochondrial ROS didn't show any significant differences among diabetic rats treated with L. album extract, diabetic control rats, and normal control rats (P=0.8). Serum glucose in diabetic control was significantly higher than normal control rats (P=0.0001). However, L. album caused a remarkable decrease in serum glucose of diabetic rats (P=0.03). Conclusion: According to the present findings, it seems that L. album at a dose of 100 mg/kg could not decrease mitochondrial ROS production from neutrophils in diabetic rats. Further studies considering higher concentrations of L. album are appreciated to evaluate its impact on the production of mitochondrial ROS along with extracellular ROS in diabetes condition. |
| نویسندگان مقاله |
کورش خانکی | Korosh Khanaki
Department of Clinical Biochemistry, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran
گروه بیوشیمی بالینی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
محمود عابدین زاده | Mahmood Abedinzade
Department of Physiology, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Science, Rasht, Iran
گروه فیزیولوژی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
روح الله گازر | Rouhollah Gazor
Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
گروه علوم تشریح، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
محمدرضا نورس فرد | Mohammadreza Norasfard
Department of Physiology, Faculty of Medicine, Guilan University of Medical Science, Rasht, Iran
گروه فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
رضا جعفری شکیب | Reza Jafari-Shakib
Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
مرکز تحقیقات سلولی و مولکولی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
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