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، جلد ۵، شماره ۱، صفحات ۲۷-۳۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Vitrification Affects Nuclear Maturation and Gene Expression of Immature Human Oocytes |
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| چکیده انگلیسی مقاله |
Background: Vitrification of oocytes is a fast-freezing technique, which may affect the quality of the human oocyte, and consequently affects the embryo development, pregnancy and birth. The aim of the current study was to investigate the consequence of in-vitro vitrification on maturation status of immature human oocytes, additionally, expression levels of stress, and apoptosis related genes. Materials and Methods: The total of 213 human immature oocytes which routinely discarded from assisted reproduction clinics were collected and divided into two groups including: (I) fresh germinal vesicle (GV) oocytes (n=106) (matured in-vitro (fIVM) , and (II) GV oocytes (n=107) that initially vitrified, then matured in in-vitro (vIVM). After 36 hours of incubation, the oocytes were evaluated for nuclear maturation and expression level of DNA methyltransferase (DNMT1), stress related genes (Sod1 and Hsp70), and apoptotic related genes (Bax and Bcl-2) by quantitative Real-Time PCR. Results: Oocyte maturation rates were reduced in vIVM compared to fIVM oocytes (P=0.001). The expression of stress (Sod1 and Hsp70), and apoptotic-related genes (Bax and Bcl-2) in vIVM were significantly higher compared to the fIVM group. Additionally, pro-apoptotic gene up-regulated 4.3 times more than anti-apoptotic gene in vIVM oocyte. However, DNMT1 gene expression was reduced in vIVM oocyte (P = 0.047). Conclusions: The low survival rate of vitrified In-vitro matured GV oocytes could definitely be explained by the alterations of their gene expression profile. |
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| کلیدواژه‌های انگلیسی مقاله |
Apoptosis, Oocytes, Vitrification |
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| نویسندگان مقاله |
عباس شاهدی | Abbas Shahedi
Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences , Yazd, Iran
احمد حسینی | Ahmad Hosseini
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
محمد علی خلیلی | Mohammad Ali Khalili
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
فرشید یگانه | Farshid Yeganeh
Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
گروه ایمونولوژی
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-617-2&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201192.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
زیست شناسی مولکولی |
| نوع مقاله منتشر شده |
پژوهشی |
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