سامانه اطلاعات پژوهشی ایران - NRIP Journals

دوشنبه 23 تیر 1404


، جلد ۵، شماره ۱، صفحات ۳۴-۳۹


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عنوان انگلیسی	Prompt cytomolecular identification of chromosome aberration in irradiated blood cells

چکیده انگلیسی مقاله	Background: understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological malignancies are determinant factors in evaluation of radiation dose and its potential in harming the body. None the less early detection of chromosomal aberration (CA) is crucial in prognosis and selection of therapy for the people exposed to irradiations. The aim of this study was to explore a swift and accurate genetic test that identifies CAs in radiologist exposed to X-rays. In addition synergistic effect of other clastogens in irradiated workers was also evaluated. Material and methods: thirty four heparinized blood samples were obtained from radiology workers exposed to X-rays. Blood samples were cultured in RPMI 1640 and F-10 Medias with and without PHA stimulation. Lymphocytes were harvested, separated and arrested at metaphase and their chromosomes were analyzed by solid and G-Banding techniques. Lymphocytic CA was also analyzed through whole chromosome painting FISH. Results: of the 37 blood sample from workers, 60% had various structural aberrations in which both the frequency and type of CAs were intensified among tobacco smokers. Conclusion: the results did not show any significant differences between the genders but other carcinogen like smoking can significantly increases the rate of CAs

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نویسندگان مقاله	محمد \| Seyed Akbar Moosavi Allied Health Department, Iran university of Medical sciences, Tehran, Iran. سید اکبر موسوی \| Zahra Ghanbari Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. زهرا قنبری \| Ayoub Farhadi Laboratory for Molecular Cytogenetic, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. ایوب فرهادی \| Fariba Heshmati Allied Health Department, Iran university of Medical sciences, Tehran, Iran. فریبا حشمتی \| Samaneh Shabani Tehran University of Medical Sciences, Tehran, Iran. \| Mohammad Ali Mofid-Nakhaei Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

نشانی اینترنتی	http://rmm.mazums.ac.ir/browse.php?a_code=A-10-880-1&slc_lang=en&sid=1
فایل مقاله	اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201193.pdf
کد مقاله (doi)
زبان مقاله منتشر شده	en
موضوعات مقاله منتشر شده	ژنتیک
نوع مقاله منتشر شده	پژوهشی

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