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، جلد ۴، شماره ۳، صفحات ۲۲-۲۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Optimization of Phenanthrene-degradation by Dietzia Cinnamea AP for Bioremediation Applications |
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| چکیده انگلیسی مقاله |
Background: This study focused on isolation and identification of a Phenanthrene (Phe) degrader bacterium and optimization of environmental conditions for Phe degradation. Materials and Methods: Enrichment technique was used for isolation and the most effective isolate; named AP was selected based on its Phe biodegradation abilities. The isolate was identified using morphological and biochemical tests as well as16S rDNA sequencing. The effects of various factors such as temperature, pH and C/N on bacterial growth and Phe degradation were investigated using protein assay (Bradford) and Gas Chromatography (GC), respectively. Results: The selected isolate was identified as Dietzia cinnamea AP. It was able to degrade Phe at pH 6-10 (optimum at 8), temperatures of 25 -45 °C (optimum at 35 °C) and NH4Cl concentrations of 0.5-2.5 gL-1 (optimum at 2 g L-1). By optimization of environmental parameters, within 10 days of fermentation, Phe degradation rate increased by more than 1.2 fold (from 60% to 73%). Conclusion: D. cinnamea AP was found to be an appropriate candidate for bioremediation applications. To the best of our knowledge, this is the first report of D. cinnamea species that can degrade Phe. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
| Zahra Fathi
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
| Gholamhossein Ebrahimipour
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
| Amirarsalan Kavyanifard
2- Department of Science, Payam Noor University (PNU), P.O. Box, 19395-3697, Tehran, Iran
| Ziba Najmi
1- Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-781-3&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201208.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
میکروبیولوژی |
| نوع مقاله منتشر شده |
پژوهشی |
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