|
|
، جلد ۳، شماره ۴، صفحات ۲۸-۳۴
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
First Detection of 16S rRNA Methylase and blaCTX-M-15 Genes among Klebsiella pneumoniae Strains Isolated from Hospitalized Patients in Iran |
|
| چکیده انگلیسی مقاله |
Background: The increasing pattern of Multi-Drug Resistant (MDR) bacteria has limited therapeutic options especially for nosocomial isolates of Klebsiella pneumoniae. Therefore, the aim of this study was the molecular detection of 16S rRNA methylase and blaCTX-M-15 among K. pneumoniae strains isolated from hospitalized patients in Mofid, Imam Hossein and Taleghani hospitals. Materials and Methods:This study was done with 110 K.pneumoniae isolated of hospitals in Tehran, Iran. Antibiotic susceptibility tests were carried out by Kirby-Bauer disc diffusion and broth microdilution methods according to CLSI guidelines. ESBL, AmpC and KPC enzymes were detected by CDDT and MHT methods and the armA, rmtB, rmtC, rmtD and blaCTX-M-15 genes were detected by PCR and sequencing techniques. Typing of antibiotic resistance isolates was carried out by PFGE technique. Results: In this study, Fosfomycin, colistin and tigecycline were more active than other antibiotics. Among the 110 K. pneumoniae strains, 60(54.5%), 33(30%) and 5(4.5%) were ESBL, Amp-C and KPC positive, respectively. The existence of blaCTX-M-15, armA and rmtC was detected in 40(36.3%), 15 (13.6%) and 2 (1.8%) respectively. Of 15 representative armA-producing K. pneumoniae isolates analyzed by PFGE, 9 different pulsotypes (PF1–9) were identified with Dice coefficients of &ge90% similarity. Conclusions: High-level aminoglycoside resistance in human pathogens result of 16S rRNA methylases is one of the serious concerns in Iran. |
|
| کلیدواژه‌های انگلیسی مقاله |
Klebsiella pneumoniae, 16s rRNA methylase , β-lactamases |
|
| نویسندگان مقاله |
| Fatemeh Ashrafian
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Fatemeh Fallah
Pediatric Infectious Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Ali Hashemi
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Soroor Erfanimanesh
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Shokouh Amraei
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
| Samira Tarashi
1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
|
|
| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-662-2&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201232.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
میکروبیولوژی |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|