، جلد ۳، شماره ۳، صفحات ۶-۱۰
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Generation of CHO Stable Cell Line Overexpressing HER2: an In Vitro Model for Breast Cancer
چکیده انگلیسی مقاله Background: Breast cancer is the most common female malignancy and the leading cause of cancer mortality in women worldwide. The human epidermal growth factor receptor2 (HER2) is a transmembrane tyrosine kinase receptor that is usually overexpressed in human breast cancers. Stable cell lines heterogeneously overexpressing HER2 are highly required as in vitro models for breast cancer research. The aim of this study was to establish a stable cell line overexpressing HER2. Materials and Methods: CHO Cells were transfected with linearized pCVN/HER2 plasmid and selected for the recombinant cells with G418 antibiotic. Expression of HER2 in the transfected cells was analyzed using western blotting and immunofluorescence. Results: We found that the recombinant cells stably expressed high levels of HER2 proteins that were mostly concentrated on the cell membrane. Conclusions: The cell line established here provides a useful in vitro model for breast cancer research and any HER-related studies.
کلیدواژه‌های انگلیسی مقاله Breast cancer, HER2, Stable cell line, Overexpression
نویسندگان مقاله | Akbar Hedayatizadeh-Omran
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.


| Reza Valadan
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran.


| Alireza Rafiei
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Abolghasem Ajami
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Mohsen Tehrani
Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran


| Reza Alizadeh-Navaei
Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
نشانی اینترنتی http://rmm.mazums.ac.ir/browse.php?a_code=A-10-777-1&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201237.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده بیولوژی
نوع مقاله منتشر شده پژوهشی
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