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عنوان انگلیسی Prevalence of mecA Gene of Methicillin Resistant Staphylococcus spp. Isolated from Nosocomial Infections and Environmental Specimens in Sanandaj Hospitals, Kurdistan, Iran
چکیده انگلیسی مقاله Background: Methicillin resistant Staphylococcus aureus (MRSA) is one of the major agents for increasing number of serious hospital and community acquired infections. The aim of this study was to investigate the occurrence of the MRSA and mecA gene among nosocomial and environmental specimens in Kurdistan hospitals and determining the antibiotic resistance of the isolates. Materials and Methods: A total of 264 clinical and environmental Staphylococcus was isolated from Kurdistan medical University Hospitals, in February 2011 to June 2012 Iran, and their susceptibility patterns to different antibiotics were determined. Furthermore, agar screen method was used to determine oxacillin resistant isolates. Finally, using PCR, the oxacillin resistant isolates were tested for the presence of mecA gene. Results: In this study, from 88 (93.18%) Staphylococcus aureus isolates, 82 were found resistant to oxacillin using agar screen method and mecA gene was detected in 66 strains (75%). Our results showed that the agar screen method is more reliable in determination of MRSA strains compared to PCR. Conclusion: In this research the studied MRSA were found with high prevalence and mecA was widespread in S. aureus isolates in Sanandaj.
کلیدواژه‌های انگلیسی مقاله Nosocomial infections, Methicillin resistance, MRSA, mecA

نویسندگان مقاله | Rashid Ramazanzadeh
Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran


| Himen Salimizand
Cellular & Molecular Research Center and Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran


| Babak Shahbazi
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran


| Masoumeh khonshah
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran


| Hanar Narenji
Microbiology Department, Member of Student Research committee, Kurdistan University of Medical Sciences, Sanandaj, Iran



نشانی اینترنتی http://rmm.mazums.ac.ir/browse.php?a_code=A-10-694-1&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201243.pdf
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زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده میکروبیولوژی
نوع مقاله منتشر شده پژوهشی
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