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، جلد ۳، شماره ۲، صفحات ۱۷-۲۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Detection of CYP2C18 m1, and m2 Alleles within an Iranian Population (Mazandaran) Using Denaturing High-Performance Liquid Chromatography (DHPLC) |
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| چکیده انگلیسی مقاله |
Background: Genetic polymorphisms of cytochrome p450 in humans are the main cause of differences in the metabolism. The allele and genotype frequencies of CYP2C19 and CYP2C9 have been studied in some Iranian populations. The aim of present study was to examine the frequencies of CYP2C18m1, and CYP2C18m2, alleles in the Mazandarani ethnic group among Iranian Population. Materials and Methods: In this study, genomic DNA was extracted from leucocytes of one hundred unrelated healthy volunteers. The prevalence of the common variants CYP2C18 m1 and m2 alleles were studied by using high fidelity polymerase chain reaction (HF-PCR) - DHPLC methods. Results: The frequency of CYP2C18 m1 and m2 alleles were 0.0% and 3.0%, respectively. CYP2C18 genotypes wt/wt, wt/m1, wt/m2, m1/m1, m1/m2, and m2/m2 frequencies were 97 %, 0.0 %, 3.0%, 0.0 %, 0.0%, and 0.0%, respectively. Conclusion: The result of the current study shows that impaired CYP2C18 activity in 03% of our sample population may decrease extra hepatic metabolism of some important drugs such as Phenytoin. It may also affect transdemal delivery of drug substrate for this isoenzyme. |
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| کلیدواژه‌های انگلیسی مقاله |
CYP2C18, Genotype, Alleles, Mazandaran, Iranian |
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| نویسندگان مقاله |
| Adeleh Rafati
Department of Nanobiotechnology, Faculty of New Sciences and Technologies, University of Isfahan, Isfahan, Iran
| Pooria Gill
Research Centre for Immunogenetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| Mehdi Shabani
Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;
| Maryam Peyrovei
Molecular and Cell Biology Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran;
| Seyed Mohammad Bagher Hashemi-Soteh
Research Centre for Immunogenetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| Mohammad-Reza Shiran
Psychiatry and Behavioral Sciences Research Centre, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-326-2&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201246.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
داروسازی |
| نوع مقاله منتشر شده |
پژوهشی |
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