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، جلد ۲، شماره ۴، صفحات ۳۳-۳۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
In vitro evaluation of capsaicin inhibitory effects on zonula occludens toxin in vibrio cholerae ATCC14035 strain |
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| چکیده انگلیسی مقاله |
Background: Cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. It is caused by certain strains of the species Vibrio cholerae which can also cause mild or in apparent infections. The aim of this study is the evaluation of Capsaicin, as a potential inhibitor of zonula occludens toxin production in V. cholerae ATCC 14035. Materials and Methods: MIC of capsaicin was determined by Broth Microdilution method according to CLSI guidelines. The zot gene expression level were analysed using real-time RT–PCR technique. Results: Results from MIC test showed that 100 μg mL−1of capsaicin was the highest concentration that did not affect the bacterial growth however, zonula occludens toxin (zot) gene expression of the tested strain was significantly inhibited by capsaicin in a dose-dependent manner at sub-bacteriocidal concentrations. The recA gene did not show any significant difference in its expression with or without capsaicin. Conclusion: Capsaicin is one of the active compounds of red chili that can drastically suppress zot gene expression and shows promising inhibitory effect against V. cholerae zot production. Thus, routine intake of red chilli, which is easily available and inexpensive, may be an alternative approach to prevent and control symptoms of cholera. |
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| کلیدواژه‌های انگلیسی مقاله |
RNA Extraction, cDNA synthesis and Real-Time PCR |
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| نویسندگان مقاله |
| Soroor Erfanimanesh
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Gita Eslami
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Hossein Goudarzi
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Arezou Taherpour
Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
| Ali Hashemi
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
| Elahe Taki
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
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| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-692-2&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201265.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بیماریهای عفونی وگرمسیری |
| نوع مقاله منتشر شده |
پژوهشی |
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