|
|
، جلد ۱، شماره ۲، صفحات ۲۳-۲۶
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژه‌های فارسی مقاله |
|
|
| عنوان انگلیسی |
A simplified protocol for producing Taq DNA polymerase in biology laboratory |
|
| چکیده انگلیسی مقاله |
Background: Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in E. coli by a reliable, practical, simple and low cost method. Materials and Methods: In this study, the Taq gene was amplified from the genomic DNA of Thermus aquaticus and cloned into pTrc99A vector. Recombinant plasmid is expressed in E. coli strain TOP10. Product protein is extracted and purified. Expression of gene was analyzed by SDS-PAGE and gene amplification. Results: SDS-PAGE showed an approximately 94 KDa protein. The density of protein bands in agarose gel electrophoresis indicated that the purified enzyme is more active than the non purified one. Conclusion: The protocols described in this paper lead to the production of pure and active enzyme that can be applied in both teaching and research laboratories. |
|
| کلیدواژه‌های انگلیسی مقاله |
Taq polymerase, expression, Purification |
|
| نویسندگان مقاله |
| Touraj Farazmandfar
Faculty of Advanced Medical Science Technologies, Golestan University of Medical Sciences, Gorgan, Iran
| Alireza Rafiei
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| Mohammad Bagher Hashemi-Sotehoh
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| Reza Valadan
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
| Mohammad Alavi
Sari Agricultural sciences & Natural Resources University, Sari, Iran
| Fatemeh Moradian
Sari Agricultural sciences & Natural Resources University, Sari, Iran
|
|
| نشانی اینترنتی |
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-9&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/2735/article-2735-2201298.pdf |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
بیوشیمی |
| نوع مقاله منتشر شده |
پژوهشی |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|