|
|
Anatomical Sciences Journal، جلد ۸، شماره ۳۲، صفحات ۰-۰
|
|
|
| عنوان فارسی |
|
|
| چکیده فارسی مقاله |
|
|
| کلیدواژههای فارسی مقاله |
|
|
| عنوان انگلیسی |
The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos |
|
| چکیده انگلیسی مقاله |
Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares' serum gonadotrophin and subsequent 5 IU/ml human chorionic gonadotrophin (ip). Embryos were cultured in T6 culture medium supplemented with different dosages of melatonin {0 (control group), 100×10-6 M, 10×10-6 M, 1×10-6 M, 100×10-9 M and 10×10-9 M (1-5 treatment groups)}. With due attention to percent of embryos in different stages, the rate of development of embryos was assessed using of invert microscope. It is also compared to control group. Results: The results showed that the rate of cleavage and development of mouse the embryos to blastocyst stage increased significantly in the development culture medium supplemented with 10 and 100 nM/mg of melatonin in comparison to control group (p< 0.001). Conclusion: The results of this study demonstrate that, enriching the culture medium with melatonin, improve mouse preimplantation development. |
|
| کلیدواژههای انگلیسی مقاله |
Melatonin, Preimplantation Embryo, Development, Mouse |
|
| نویسندگان مقاله |
| Zakieh Asgari
| Mina Ramezani
| Mohammad Hadi Bahadori
| Masoumeh Ahmadi Jalali Moghaddam
|
|
| نشانی اینترنتی |
http://anatomyjournal.ir/browse.php?a_code=A-10-304-214&slc_lang=fa&sid=1 |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
|
| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
Morphometry |
| نوع مقاله منتشر شده |
Original |
|
|
|
برگشت به:
صفحه اول پایگاه |
نسخه مرتبط |
نشریه مرتبط |
فهرست نشریات
|